Human ENPP1 ELISA Kit

Alternative name
Human E-NPP 1 ELISA Kit;Human Membrane component chromosome 6 surface marker 1 ELISA Kit;Human phosphodiesterase I/nucleotide pyrophosphatase 1 ELISA Kit;Human plasma-cell membrane glycoprotein PC-1 ELISA Kit;Human NPPase ELISA Kit;Human Nucleotide diphosphatase ELISA Kit;Human M6S1 ELISA Kit;Human NPPS ELISA Kit;Human PC1 ELISA Kit;Human PDNP1 ELISA Kit;Human ARHR2 ELISA Kit;Human COLED ELISA Kit;Human NPP1 ELISA Kit;Human PC-1 ELISA Kit;Human PCA1 ELISA Kit;Human ectonucleotide pyrophosphatase/phosphodiesterase 1 ELISA Kit;Human ectonucleotide pyrophosphatase/phosphodiesterase family member 1 ELISA Kit;Human Ly-41 antigen ELISA Kit;Human alkaline phosphodiesterase 1 ELISA Kit;Human membrane component, chromosome 6, surface marker 1 ELISA Kit;Human plasma-cell membrane glycoprotein 1 ELISA Kit;
Catalog
E012751
species
Human
GeneENPP1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1). No significant cross-reactivity or interference between Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.062ng/mL.
Intended UseHuman ENPP1 ELISA Kit allows for the in vitro quantitative determination of ENPP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Nucleotide pyrophosphatase Protein information
Uniprot ID ENPP1_HUMAN
Uniprot AC P22413; Q5T9R6; Q9NPZ3; Q9P1P6; Q9UP61; Q9Y6K3;
UniGene Hs.527295;
GeneID 5167
KEGG hsa:5167;
Human Nucleotide pyrophosphatase Protein SEQUENCE
SEQUENCE 925 AA; 104924 MW; 0ECAA063801CAFEB CRC64;
MERDGCAGGG SRGGEGGRAP REGPAGNGRD RGRSHAAEAP GDPQAAASLL
APMDVGEEPL EKAARARTAK DPNTYKVLSL VLSVCVLTTI LGCIFGLKPS
CAKEVKSCKG RCFERTFGNC RCDAACVELG NCCLDYQETC IEPEHIWTCN
KFRCGEKRLT RSLCACSDDC KDKGDCCINY SSVCQGEKSW VEEPCESINE
PQCPAGFETP PTLLFSLDGF RAEYLHTWGG LLPVISKLKK CGTYTKNMRP
VYPTKTFPNH YSIVTGLYPE SHGIIDNKMY DPKMNASFSL KSKEKFNPEW
YKGEPIWVTA KYQGLKSGTF FWPGSDVEIN GIFPDIYKMY NGSVPFEERI
LAVLQWLQLP KDERPHFYTL YLEEPDSSGH SYGPVSSEVI KALQRVDGMV
GMLMDGLKEL NLHRCLNLIL ISDHGMEQGS CKKYIYLNKY LGDVKNIKVI
YGPAARLRPS DVPDKYYSFN YEGIARNLSC REPNQHFKPY LKHFLPKRLH
FAKSDRIEPL TFYLDPQWQL ALNPSERKYC GSGFHGSDNV FSNMQALFVG
YGPGFKHGIE ADTFENIEVY NLMCDLLNLT PAPNNGTHGS LNHLLKNPVY
TPKHPKEVHP LVQCPFTRNP RDNLGCSCNP SILPIEDFQT QFNLTVAEEK
IIKHETLPYG RPRVLQKENT ICLLSQHQFM SGYSQDILMP LWTSYTVDRN
DSFSTEDFSN CLYQDFRIPL SPVHKCSFYK NNTKVSYGFL SPPQLNKNSS
GIYSEALLTT NIVPMYQSFQ VIWRYFHDTL LRKYAEERNG VNVVSGPVFD
FDYDGRCDSL ENLRQKRRVI RNQEILIPTH FFIVLTSCKD TSQTPLHCEN
LDTLAFILPH RTDNSESCVH GKHDSSWVEE LLMLHRARIT DVEHITGLSF
YQQRKEPVSD ILKLKTHLPT FSQED
UCSC uc011ecf.2; human.;

Category

Human ENPP1 ELISA Kit