Human EGLN1 ELISA Kit

Alternative name
Human hypoxia-inducible factor prolyl hydroxylase 2 ELISA Kit;Human HIF-PH2 ELISA Kit;Human HIF-prolyl hydroxylase 2 ELISA Kit;Human HPH-2 ELISA Kit;Human prolyl hydroxylase domain-containing protein 2 ELISA Kit;Human PHD2 ELISA Kit;Human SM-20 ELISA Kit;Human C1orf12 ELISA Kit;Human ECYT3 ELISA Kit;Human HALAH ELISA Kit;Human HIFPH2 ELISA Kit;Human HPH2 ELISA Kit;Human SM20 ELISA Kit;Human ZMYND6 ELISA Kit;Human egl-9 family hypoxia inducible factor 1 ELISA Kit;Human egl nine homolog 1 ELISA Kit;Human egl nine-like protein 1 ELISA Kit;Human zinc finger MYND domain-containing protein 6 ELISA Kit;
Catalog
E012831
species
Human
GeneEGLN1
SpecificityThis assay has high sensitivity and excellent specificity for detection of human EGLN1/C1ORF12/PNAS-118/PNAS-137. No significant cross-reactivity or interference between human EGLN1/C1ORF12/PNAS-118/PNAS-137 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human EGLN1/C1ORF12/PNAS-118/PNAS-137 is typically less than 7.8 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero.
Intended UseHuman EGLN1 ELISA Kit allows for the in vitro quantitative determination of EGLN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EGLN1/C1ORF12/PNAS-118/PNAS-137 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EGLN1/C1ORF12/PNAS-118/PNAS-137 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGLN1/C1ORF12/PNAS-118/PNAS-137 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGLN1/C1ORF12/PNAS-118/PNAS-137 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Egl nine homolog 1 Protein information
Uniprot ID EGLN1_HUMAN
Uniprot AC YPGNGTGY VRHVDNPNGD GRCVTCIYYL NKDWDAKVSG GILRIFPEGK AQFADIEPKF
UniGene Hs.444450;
GeneID 54583
KEGG hsa:54583;
Human Egl nine homolog 1 Protein SEQUENCE
SEQUENCE 426 AA; 46021 MW; 81A97FF772CAA14C CRC64;
MANDSGGPGG PSPSERDRQY CELCGKMENL LRCSRCRSSF YCCKEHQRQD
WKKHKLVCQG SEGALGHGVG PHQHSGPAPP AAVPPPRAGA REPRKAAARR
DNASGDAAKG KVKAKPPADP AAAASPCRAA AGGQGSAVAA EAEPGKEEPP
ARSSLFQEKA NLYPPSNTPG DALSPGGGLR PNGQTKPLPA LKLALEYIVP
CMNKHGICVV DDFLGKETGQ QIGDEVRALH DTGKFTDGQL VSQKSDSSKD
IRGDKITWIE GKEPGCETIG LLMSSMDDLI RHCNGKLGSY KINGRTKAMV
ACYPGNGTGY VRHVDNPNGD GRCVTCIYYL NKDWDAKVSG GILRIFPEGK
AQFADIEPKF DRLLFFWSDR RNPHEVQPAY ATRYAITVWY FDADERARAK
VKYLTGEKGV RVELNKPSDS VGKDVF

Category

Human EGLN1 ELISA Kit