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Human ELN ELISA Kit

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  • Alternative name

    Human tropoelastin ELISA Kit;Human SVAS ELISA Kit;Human WBS ELISA Kit;Human WS ELISA Kit;Human elastin ELISA Kit;
  • Catalog
    E012883
  • species
    Human
  • GeneELN
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ELN. No significant cross-reactivity or interference between ELN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ELN and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ELN ELISA Kit allows for the in vitro quantitative determination of ELN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical    Principle of the assay: ELN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ELN antibody and an ELN-HRP conjugate. The assay sample and buffer are incubated together with ELN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ELN concentration since ELN from samples and ELN-HRP conjugate compete for the anti-ELN antibody binding site. Since the number of sites is limited, as more sites are occupied by ELN from the sample, fewer sites are left to bind ELN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ELN concentration in each sample is interpolated from this standard curve.
  • Human Elastin Protein information
  • Uniprot ID ELN_HUMAN
  • Uniprot AC Q6P0L4; Q6ZWJ6; Q75MU5; Q7Z316; Q7Z3F5; Q9UMF5;
  • UniGene Hs.647061;
  • GeneID 2006
  • KEGG hsa:2006;
  • Human Elastin Protein SEQUENCE

  • SEQUENCE 786 AA; 68469 MW; D3C6F4EC6BC4991F CRC64;

    MAGLTAAAPR PGVLLLLLSI LHPSRPGGVP GAIPGGVPGG VFYPGAGLGA

    LGGGALGPGG KPLKPVPGGL AGAGLGAGLG AFPAVTFPGA LVPGGVADAA

    AAYKAAKAGA GLGGVPGVGG LGVSAGAVVP QPGAGVKPGK VPGVGLPGVY

    PGGVLPGARF PGVGVLPGVP TGAGVKPKAP GVGGAFAGIP GVGPFGGPQP

    GVPLGYPIKA PKLPGGYGLP YTTGKLPYGY GPGGVAGAAG KAGYPTGTGV

    GPQAAAAAAA KAAAKFGAGA AGVLPGVGGA GVPGVPGAIP GIGGIAGVGT

    PAAAAAAAAA AKAAKYGAAA GLVPGGPGFG PGVVGVPGAG VPGVGVPGAG

    IPVVPGAGIP GAAVPGVVSP EAAAKAAAKA AKYGARPGVG VGGIPTYGVG

    AGGFPGFGVG VGGIPGVAGV PGVGGVPGVG GVPGVGISPE AQAAAAAKAA

    KYGAAGAGVL GGLVPGPQAA VPGVPGTGGV PGVGTPAAAA AKAAAKAAQF

    GLVPGVGVAP GVGVAPGVGV APGVGLAPGV GVAPGVGVAP GVGVAPGIGP

    GGVAAAAKSA AKVAAKAQLR AAAGLGAGIP GLGVGVGVPG LGVGAGVPGL

    GVGAGVPGFG AGADEGVRRS LSPELREGDP SSSQHLPSTP SSPRVPGALA

    AAKAAKYGAA VPGVLGGLGA LGGVGIPGGV VGAGPAAAAA AAKAAAKAAQ

    FGLVGAAGLG GLGVGGLGVP GVGGLGGIPP AAAAKAAKYG AAGLGGVLGG

    AGQFPLGGVA ARPGFGLSPI FPGGACLGKA CGRKRK

  • UCSC uc003tzn.5; human. [P15502-3];



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