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Human ETFA ELISA Kit

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  • Alternative name

    Human alpha-ETF ELISA Kit;Human EMA ELISA Kit;Human GA2 ELISA Kit;Human MADD ELISA Kit;Human electron transfer flavoprotein alpha subunit ELISA Kit;Human electron transfer flavoprotein subunit alpha, mitochondrial ELISA Kit;Human electron transfer flavoprotein, alpha polypeptide ELISA Kit;Human glutaric aciduria II ELISA Kit;
  • Catalog
    E012904
  • species
    Human
  • GeneETFA
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Electron Transfer Flavoprotein Alpha Polypeptide (ETFa). No significant cross-reactivity or interference between Electron Transfer Flavoprotein Alpha Polypeptide (ETFa) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.056ng/mL.
  • Intended UseHuman ETFA ELISA Kit allows for the in vitro quantitative determination of ETFA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Electron Transfer Flavoprotein Alpha Polypeptide (ETFa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Electron Transfer Flavoprotein Alpha Polypeptide (ETFa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Electron Transfer Flavoprotein Alpha Polypeptide (ETFa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Electron Transfer Flavoprotein Alpha Polypeptide (ETFa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Electron transfer flavoprotein subunit alpha, mitochondrial Protein information
  • Uniprot ID ETFA_HUMAN
  • Uniprot AC P13804; B4DT43; Q53XN3;
  • UniGene Hs.39925;
  • GeneID 2108
  • KEGG hsa:2108;
  • Human Electron transfer flavoprotein subunit alpha, mitochondrial Protein SEQUENCE

  • SEQUENCE 333 AA; 35080 MW; 2EC6D1ADE68CBDB5 CRC64;

    MFRAAAPGQL RRAASLLRFQ STLVIAEHAN DSLAPITLNT ITAATRLGGE

    VSCLVAGTKC DKVAQDLCKV AGIAKVLVAQ HDVYKGLLPE ELTPLILATQ

    KQFNYTHICA GASAFGKNLL PRVAAKLEVA PISDIIAIKS PDTFVRTIYA

    GNALCTVKCD EKVKVFSVRG TSFDAAATSG GSASSEKASS TSPVEISEWL

    DQKLTKSDRP ELTGAKVVVS GGRGLKSGEN FKLLYDLADQ LHAAVGASRA

    AVDAGFVPND MQVGQTGKIV APELYIAVGI SGAIQHLAGM KDSKTIVAIN

    KDPEAPIFQV ADYGIVADLF KVVPEMTEIL KKK

  • UCSC uc002bbt.3; human. [P13804-1];



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