Human ELOA ELISA Kit

Alternative name
Human EloA ELISA Kit;Human elongin 110 kDa subunit ELISA Kit;Human RNA polymerase II transcription factor SIII subunit A1 ELISA Kit;Human SIII p110 ELISA Kit;Human transcription elongation factor B polypeptide 3 ELISA Kit;Human TCEB3 ELISA Kit;Human SIII ELISA Kit;Human TCEB3A ELISA Kit;Human elongin A ELISA Kit;Human transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A) ELISA Kit;Human transcription elongation factor B alpha subunit ELISA Kit;Human transcription elongation factor B subunit 3 ELISA Kit;
Catalog
E012934
species
Human
GeneELOA
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human EloA. No significant cross-reactivity or interference between Human EloA and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human EloA and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman ELOA ELISA Kit allows for the in vitro quantitative determination of ELOA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EloA in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human EloA. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human EloA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EloA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human EloA. You can calculate the concentration of Human EloA in the samples by comparing the OD of the samples to the standard curve.
Human Elongin-A Protein information
Uniprot ID ELOA1_HUMAN
Uniprot AC Q14241; B2R7Q8; Q8IXH1;
UniGene Hs.15535;
GeneID 6924
KEGG hsa:6924;
Human Elongin-A Protein SEQUENCE
SEQUENCE 798 AA; 89909 MW; 2C8FD8710E27EE46 CRC64;
MHGGRSCGPR TRREPSSGEE AAPVTAMAAE SALQVVEKLQ ARLAANPDPK
KLLKYLKKLS TLPITVDILA ETGVGKTVNS LRKHEHVGSF ARDLVAQWKK
LVPVERNAEP DEQDFEKSNS RKRPRDALQK EEEMEGDYQE TWKATGSRSY
SPDHRQKKHR KLSELERPHK VSHGHERRDE RKRCHRMSPT YSSDPESSDY
GHVQSPPSCT SPHQMYVDHY RSLEEDQEPI VSHQKPGKGH SNAFQDRLGA
SQERHLGEPH GKGVVSQNKE HKSSHKDKRP VDAKSDEKAS VVSREKSHKA
LSKEENRRPP SGDNAREKPP SSGVKKEKDR EGSSLKKKCL PPSEAASDNH
LKKPKHRDPE KAKLDKSKQG LDSFDTGKGA GDLLPKVKEK GSNNLKTPEG
KVKTNLDRKS LGSLPKVEET DMEDEFEQPT MSFESYLSYD QPRKKKKKIV
KTSATALGDK GLKKNDSKST GKNLDSVQKL PKVNKTKSEK PAGADLAKLR
KVPDVLPVLP DLPLPAIQAN YRPLPSLELI SSFQPKRKAF SSPQEEEEAG
FTGRRMNSKM QVYSGSKCAY LPKMMTLHQQ CIRVLKNNID SIFEVGGVPY
SVLEPVLERC TPDQLYRIEE YNHVLIEETD QLWKVHCHRD FKEERPEEYE
SWREMYLRLQ DAREQRLRVL TKNIQFAHAN KPKGRQAKMA FVNSVAKPPR
DVRRRQEKFG TGGAAVPEKI KIKPAPYPMG SSHASASSIS FNPSPEEPAY
DGPSTSSAHL APVVSSTVSY DPRKPTVKKI APMMAKTIKA FKNRFSRR
UCSC uc001bho.4; human.;

Category

Human ELOA ELISA Kit