Human EBP ELISA Kit

Alternative name
Human cholestenol Delta-isomerase ELISA Kit;Human delta(8)-Delta(7) sterol isomerase ELISA Kit;Human D8-D7 sterol isomerase ELISA Kit;Human emopamil-binding protein ELISA Kit;Human CDPX2 ELISA Kit;Human CHO2 ELISA Kit;Human CPX ELISA Kit;Human CPXD ELISA Kit;Human MEND ELISA Kit;Human emopamil binding protein (sterol isomerase) ELISA Kit;Human 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase ELISA Kit;Human 3-beta-hydroxysteroid-delta-8,delta-7-isomerase ELISA Kit;Human Chondrodysplasia punctata-2, X-linked dominant (Happle syndrome) ELISA Kit;Human sterol 8-isomerase ELISA Kit;
Catalog
E012988
species
Human
GeneEBP
SpecificityThis assay has high sensitivity and excellent specificity for detection of human EBP. No significant cross-reactivity or interference between human EBP and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human EBP is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
Intended UseHuman EBP ELISA Kit allows for the in vitro quantitative determination of EBP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EBP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EBP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase Protein information
Uniprot ID EBP_HUMAN
Uniprot AC Q15125; Q6FGL3; Q6IBI9;
UniGene Hs.30619;
GeneID 10682
KEGG hsa:10682;
Human 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase Protein SEQUENCE
SEQUENCE 230 AA; 26353 MW; 3931A9DE3DBAFA04 CRC64;
MTTNAGPLHP YWPQHLRLDN FVPNDRPTWH ILAGLFSVTG VLVVTTWLLS
GRAAVVPLGT WRRLSLCWFA VCGFIHLVIE GWFVLYYEDL LGDQAFLSQL
WKEYAKGDSR YILGDNFTVC METITACLWG PLSLWVVIAF LRQHPLRFIL
QLVVSVGQIY GDVLYFLTEH RDGFQHGELG HPLYFWFYFV FMNALWLVLP
GVLVLDAVKH LTHAQSTLDA KATKAKSKKN
UCSC uc004djx.5; human.;

Category

Human EBP ELISA Kit