Human ENGASE ELISA Kit

Alternative name
Human ENGase ELISA Kit;Human endo-beta-N-acetylglucosaminidase ELISA Kit;Human cytosolic endo-beta-N-acetylglucosaminidase ELISA Kit;Human Di-N-acetylchitobiosyl beta-N-acetylglucosaminidase ELISA Kit;Human Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase ELISA Kit;
Catalog
E013004
species
Human
GeneENGASE
SpecificityThis assay has high sensitivity and excellent specificity for detection of Endo Beta-N-Acetylglucosaminidase (ENGASE). No significant cross-reactivity or interference between Endo Beta-N-Acetylglucosaminidase (ENGASE) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.126ng/mL.
Intended UseHuman ENGASE ELISA Kit allows for the in vitro quantitative determination of ENGASE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endo Beta-N-Acetylglucosaminidase (ENGASE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endo Beta-N-Acetylglucosaminidase (ENGASE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endo Beta-N-Acetylglucosaminidase (ENGASE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Endo Beta-N-Acetylglucosaminidase (ENGASE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Cytosolic endo-beta-N-acetylglucosaminidase Protein information
Uniprot ID ENASE_HUMAN
Uniprot AC Q8NFI3; Q659F0; Q8TB86; Q9H6U4;
UniGene Hs.29288;
GeneID 64772
KEGG hsa:64772;
Human Cytosolic endo-beta-N-acetylglucosaminidase Protein SEQUENCE
SEQUENCE 743 AA; 83987 MW; 384DA533AE549FC5 CRC64;
MEAAAVTVTR SATRRRRRQL QGLAAPEAGT QEEQEDQEPR PRRRRPGRSI
KDEEEETVFR EVVSFSPDPL PVRYYDKDTT KPISFYLSSL EELLAWKPRL
EDGFNVALEP LACRQPPLSS QRPRTLLCHD MMGGYLDDRF IQGSVVQTPY
AFYHWQCIDV FVYFSHHTVT IPPVGWTNTA HRHGVCVLGT FITEWNEGGR
LCEAFLAGDE RSYQAVADRL VQITQFFRFD GWLINIENSL SLAAVGNMPP
FLRYLTTQLH RQVPGGLVLW YDSVVQSGQL KWQDELNQHN RVFFDSCDGF
FTNYNWREEH LERMLGQAGE RRADVYVGVD VFARGNVVGG RFDTDKSLEL
IRKHGFSVAL FAPGWVYECL EKKDFFQNQD KFWGRLERYL PTHSICSLPF
VTSFCLGMGA RRVCYGQEEA VGPWYHLSAQ EIQPLFGEHR LGGDGRGWVR
THCCLEDAWH GGSSLLVRGV IPPEVGNVAV RLFSLQAPVP PKIYLSMVYK
LEGPTDVTVA LELTTGDAGS CHIGGISVLN AETSSRHSLR PLRVPPTKLA
RWVGRCGRQL SGGWVQHCYE VSLRGCLLLD LLVCFSRPPG SREEESFTCR
LGEIQVVDAA SLLAPLPQVQ AVTISHIRWQ PSASEREGPP ALLQLSCTLH
WSFLLSQVRC FRIHCWGGMS DDSPGRELPR PEMPMFLGLA FATQYRIVDL
LVEAAGPGQD RRMEFLVEPV PKEGFRVPQA EWGRAVLLYS APA
UCSC uc002jwv.5; human. [Q8NFI3-1];

Category

Human ENGASE ELISA Kit

Human ENGase ELISA Kit;Human endo-beta-N-acetylglucosaminidase ELISA Kit;Human cytosolic endo-beta-N-acetylglucosaminidase ELISA Kit;Human Di-N-acetylchitobiosyl beta-N-acetylglucosaminidase ELISA Kit;Human Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase ELISA Kit;