Human ESAM ELISA Kit

Alternative name
Human W117m ELISA Kit;Human endothelial cell adhesion molecule ELISA Kit;Human endothelial cell-selective adhesion molecule ELISA Kit;Human 2310008D05Rik ELISA Kit;Human HUEL (C4orf1)-interacting protein ELISA Kit;Human LP4791 protein ELISA Kit;
Catalog
E013075
species
Human
GeneESAM
SpecificityThis assay has high sensitivity and excellent specificity for detection of Endothelial Cell Adhesion Molecule (ESAM). No significant cross-reactivity or interference between Endothelial Cell Adhesion Molecule (ESAM) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.116ng/mL.
Intended UseHuman ESAM ELISA Kit allows for the in vitro quantitative determination of ESAM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endothelial Cell Adhesion Molecule (ESAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endothelial Cell Adhesion Molecule (ESAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endothelial Cell Adhesion Molecule (ESAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Endothelial Cell Adhesion Molecule (ESAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Endothelial cell-selective adhesion molecule Protein information
Uniprot ID ESAM_HUMAN
Uniprot AC Q96AP7; B4DVN8; Q96T50;
UniGene Hs.173840;
GeneID 90952
KEGG hsa:90952;
Human Endothelial cell-selective adhesion molecule Protein SEQUENCE
SEQUENCE 390 AA; 41176 MW; C5E3F302F41B6EEC CRC64;
MISLPGPLVT NLLRFLFLGL SALAPPSRAQ LQLHLPANRL QAVEGGEVVL
PAWYTLHGEV SSSQPWEVPF VMWFFKQKEK EDQVLSYING VTTSKPGVSL
VYSMPSRNLS LRLEGLQEKD SGPYSCSVNV QDKQGKSRGH SIKTLELNVL
VPPAPPSCRL QGVPHVGANV TLSCQSPRSK PAVQYQWDRQ LPSFQTFFAP
ALDVIRGSLS LTNLSSSMAG VYVCKAHNEV GTAQCNVTLE VSTGPGAAVV
AGAVVGTLVG LGLLAGLVLL YHRRGKALEE PANDIKEDAI APRTLPWPKS
SDTISKNGTL SSVTSARALR PPHGPPRPGA LTPTPSLSSQ ALPSPRLPTT
DGAHPQPISP IPGGVSSSGL SRMGAVPVMV PAQSQAGSLV
UCSC uc001qav.5; human. [Q96AP7-1];

Category

Human ESAM ELISA Kit

Human W117m ELISA Kit;Human endothelial cell adhesion molecule ELISA Kit;Human endothelial cell-selective adhesion molecule ELISA Kit;Human 2310008D05Rik ELISA Kit;Human HUEL (C4orf1)-interacting protein ELISA Kit;Human LP4791 protein ELISA Kit;