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Alternative name
Nitric oxide synthase, endothelial ELISA KIT; Constitutive NOS ELISA KIT; cNOS ELISA KIT; EC-NOS ELISA KIT; Endothelial NOS ELISA KIT; eNOS ELISA KIT; NOS type III ELISA KIT; NOSIIINOS3 ELISA KIT; cNOS ELISA KIT; eNOS ELISA KIT; NOSIII ELISA KIT
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Catalog
E013089
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species
Human
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GeneeNOS
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SpecificityNo cross-reaction with other factors.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityUp to 0.05 ng/ml.
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Intended UseHuman eNOS ELISA Kit allows for the in vitro quantitative determination of eNOS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human eNOS monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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