Human, Mouse, Rat, Cow, Dog, Pig and Rabbit EDN1 Elisa Kit
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Alternative name
Endothelin-1 ELISA KIT; Preproendothelin-1 ELISA KIT; PPET1EDN1 ELISA KIT; PPET1 ELISA KIT; ET-1 ELISA KIT
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Catalog
E013137
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species
Human, Mouse, Rat, Cow, Dog, Pig and Rabbit
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GeneEDN1
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseHuman, Mouse, Rat, Cow, Dog, Pig and Rabbit EDN1 Elisa Kit allows for the in vitro quantitative determination of EDN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyCytokines, Growth Factors & Hormones; Elisa Kits
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Product Description
specificalProduct Information: Endothelin-1 (ET-1) ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of human, mouse, rat, cow, dog, pig and rabbit ET-1. Sensitivity: 0.4 pg/ml (range: 0.78-100 pg/ml). 100 assays.
Background: Biobool's Endothelin-1 (ET-1) ELISA kit is a competitive immunoassay for the quantitative determination of Endothelin-1 in biological fluids. ET-1 has identical amino acid sequence for human, mouse, rat, cow, dog, pig, and rabbit. The basal circulating level of ET-1 is reported to be < 1 to 3pg/mL but is known to be elevated in atrial and pulmonary hypertensions, atherosclerosis, congestive heart failure, cancer and variably related to lung diseases such as COPD and asthma. Samples and standards are added to wells coated with a monoclonal antibody specific for ET-1 and incubated. The plate is washed, leaving only bound ET-1 on the plate. A solution of HRP labeled monoclonal antibody to ET-1 is then added. This binds the ET-1 captured on the plate. The plate is then incubated. The plate is washed to remove excess HRP labeled antibody. TMB Substrate solution is added. The substrate generates a blue color when catalyzed by the HRP. Stop solution is added to stop the substrate reaction. The resulting yellow color is read at 450 nm. The amount of signal is directly proportional to the level of ET-1 in the sample. Sensitivity: 0.4 pg/ml (range: 0.78-100 pg/ml). The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. Cross Reactivity: ET-1: 100%, ET-2: 21%, ET-3: 3.6%, human Big ET-1: <0.1%, rat Big ET-1: <0.1%, human Big ET-2: <0.1%, human Big ET-3 amide: <0.1%.
Summary: Detection method- Absorbance (450 nm)
Species reactivity- Human, mouse, rat, cow, dog, pig and rabbit
Application- Quantitative detection of Endothelin-1
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