Human EZH2 ELISA Kit

Alternative name
Human ENX-1 ELISA Kit;Human enhancer of zeste homolog 2 ELISA Kit;Human lysine N-methyltransferase 6 ELISA Kit;Human KMT6 ELISA Kit;Human ENX1 ELISA Kit;Human EZH1 ELISA Kit;Human EZH2b ELISA Kit;Human KMT6A ELISA Kit;Human WVS ELISA Kit;Human WVS2 ELISA Kit;Human enhancer of zeste 2 polycomb repressive complex 2 subunit ELISA Kit;Human histone-lysine N-methyltransferase EZH2 ELISA Kit;
Catalog
E013173
species
Human
GeneEZH2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Enhancer Of Zeste Homolog 2 (EZH2). No significant cross-reactivity or interference between Enhancer Of Zeste Homolog 2 (EZH2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.057ng/mL.
Intended UseHuman EZH2 ELISA Kit allows for the in vitro quantitative determination of EZH2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Enhancer Of Zeste Homolog 2 (EZH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Enhancer Of Zeste Homolog 2 (EZH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Enhancer Of Zeste Homolog 2 (EZH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Enhancer Of Zeste Homolog 2 (EZH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Histone-lysine N-methyltransferase EZH2 Protein information
Uniprot ID EZH2_HUMAN
Uniprot AC Q96FI6;
UniGene Hs.444082; Hs.732308;
GeneID 2146
KEGG hsa:2146;
Human Histone-lysine N-methyltransferase EZH2 Protein SEQUENCE
SEQUENCE 746 AA; 85363 MW; 1B5029EB9D509BE5 CRC64;
MGQTGKKSEK GPVCWRKRVK SEYMRLRQLK RFRRADEVKS MFSSNRQKIL
ERTEILNQEW KQRRIQPVHI LTSVSSLRGT RECSVTSDLD FPTQVIPLKT
LNAVASVPIM YSWSPLQQNF MVEDETVLHN IPYMGDEVLD QDGTFIEELI
KNYDGKVHGD RECGFINDEI FVELVNALGQ YNDDDDDDDG DDPEEREEKQ
KDLEDHRDDK ESRPPRKFPS DKIFEAISSM FPDKGTAEEL KEKYKELTEQ
QLPGALPPEC TPNIDGPNAK SVQREQSLHS FHTLFCRRCF KYDCFLHPFH
ATPNTYKRKN TETALDNKPC GPQCYQHLEG AKEFAAALTA ERIKTPPKRP
GGRRRGRLPN NSSRPSTPTI NVLESKDTDS DREAGTETGG ENNDKEEEEK
KDETSSSSEA NSRCQTPIKM KPNIEPPENV EWSGAEASMF RVLIGTYYDN
FCAIARLIGT KTCRQVYEFR VKESSIIAPA PAEDVDTPPR KKKRKHRLWA
AHCRKIQLKK DGSSNHVYNY QPCDHPRQPC DSSCPCVIAQ NFCEKFCQCS
SECQNRFPGC RCKAQCNTKQ CPCYLAVREC DPDLCLTCGA ADHWDSKNVS
CKNCSIQRGS KKHLLLAPSD VAGWGIFIKD PVQKNEFISE YCGEIISQDE
ADRRGKVYDK YMCSFLFNLN NDFVVDATRK GNKIRFANHS VNPNCYAKVM
MVNGDHRIGI FAKRAIQTGE ELFFDYRYSQ ADALKYVGIE REMEIP
UCSC uc003wfb.3; human. [Q15910-1];

Category

Human EZH2 ELISA Kit