Human EN2 ELISA Kit

Alternative name
Human homeobox protein en-2 ELISA Kit;Human hu-En-2 ELISA Kit;Human engrailed homeobox 2 ELISA Kit;Human homeobox protein engrailed-2 ELISA Kit;Human engrailed homolog 2 ELISA Kit;Human engrailed-2 ELISA Kit;
Catalog
E013181
species
Human
GeneEN2
SpecificityThis assay has high sensitivity and excellent specificity for detection of EN2. No significant cross-reactivity or interference between EN2 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.118ng/mL
Intended UseHuman EN2 ELISA Kit allows for the in vitro quantitative determination of EN2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EN2 in human tissue homogenates and other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to EN2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to EN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm Â± 10nm. The concentration of EN2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Homeobox protein engrailed-2 Protein information
Uniprot ID HME2_HUMAN
Uniprot AC P19622; A4D252; Q549U3; Q9UD58;
UniGene Hs.134989;
GeneID 2020
KEGG hsa:2020;
Human Homeobox protein engrailed-2 Protein SEQUENCE
SEQUENCE 333 AA; 34211 MW; ACF5399E383D6257 CRC64;
MEENDPKPGE AAAAVEGQRQ PESSPGGGSG GGGGSSPGEA DTGRRRALML
PAVLQAPGNH QHPHRITNFF IDNILRPEFG RRKDAGTCCA GAGGGRGGGA
GGEGGASGAE GGGGAGGSEQ LLGSGSREPR QNPPCAPGAG GPLPAAGSDS
PGDGEGGSKT LSLHGGAKKG GDPGGPLDGS LKARGLGGGD LSVSSDSDSS
QAGANLGAQP MLWPAWVYCT RYSDRPSSGP RSRKPKKKNP NKEDKRPRTA
FTAEQLQRLK AEFQTNRYLT EQRRQSLAQE LSLNESQIKI WFQNKRAKIK
KATGNKNTLA VHLMAQGLYN HSTTAKEGKS DSE
UCSC uc003wmb.3; human.;

Category

Human EN2 ELISA Kit

Human homeobox protein en-2 ELISA Kit;Human hu-En-2 ELISA Kit;Human engrailed homeobox 2 ELISA Kit;Human homeobox protein engrailed-2 ELISA Kit;Human engrailed homolog 2 ELISA Kit;Human engrailed-2 ELISA Kit;