Human ENO1 ELISA Kit

Alternative name
Human 2-phospho-D-glycerate hydro-lyase ELISA Kit;Human C-myc promoter-binding protein ELISA Kit;Human enolase 1 ELISA Kit;Human MBP-1 ELISA Kit;Human MPB-1 ELISA Kit;Human non-neural enolase ELISA Kit;Human NNE ELISA Kit;Human phosphopyruvate hydratase ELISA Kit;Human plasminogen-binding protein ELISA Kit;Human ENO1L1 ELISA Kit;Human MBPB1 ELISA Kit;Human MPB1 ELISA Kit;Human HEL-S-17 ELISA Kit;Human PPH ELISA Kit;Human alpha-enolase ELISA Kit;Human c-myc promoter-binding protein-1 ELISA Kit;Human MYC promoter-binding protein 1 ELISA Kit;Human alpha enolase like 1 ELISA Kit;Human enolase 1, (alpha) ELISA Kit;Human enolase-alpha ELISA Kit;Human epididymis secretory protein Li 17 ELISA Kit;Human tau-crystallin ELISA Kit;
Catalog
E013195
species
Human
GeneENO1
SpecificityThis assay has high sensitivity and excellent specificity for detection of human ENO1/ENO1L1/MBPB1/MPB1. No significant cross-reactivity or interference between human ENO1/ENO1L1/MBPB1/MPB1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human ENO1/ENO1L1/MBPB1/MPB1 is typically less than 0.31 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was
Intended UseHuman ENO1 ELISA Kit allows for the in vitro quantitative determination of ENO1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ENO1/ENO1L1/MBPB1/MPB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ENO1/ENO1L1/MBPB1/MPB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ENO1/ENO1L1/MBPB1/MPB1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ENO1/ENO1L1/MBPB1/MPB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Alpha-enolase Protein information
Uniprot ID ENOA_HUMAN
Uniprot AC Q6GMP2; Q71V37; Q7Z3V6; Q8WU71; Q96GV1; Q9BT62; Q9UCH6; Q9UM55;
UniGene Hs.517145;
GeneID 2023
KEGG hsa:2023;
Human Alpha-enolase Protein SEQUENCE
SEQUENCE 434 AA; 47169 MW; A0ED663FCC15ADA5 CRC64;
MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR
DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT
ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF
NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY
GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE
FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD
WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV
TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR
SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK
UCSC uc001apj.3; human. [P06733-1];

Category

Human ENO1 ELISA Kit