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Human ECH1 ELISA Kit

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  • Alternative name

    Human HPXEL ELISA Kit;Human enoyl-CoA hydratase 1 ELISA Kit;Human delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial ELISA Kit;Human delta3,5-delta2,4-dienoyl-CoA isomerase ELISA Kit;Human delta3,5-delta2,4-dienoyl-coenzyme A isomerase ELISA Kit;Human dienoyl-CoA isomerase ELISA Kit;Human enoyl Coenzyme A hydratase 1, peroxisomal ELISA Kit;Human enoyl-CoA hydratase 1, peroxisomal ELISA Kit;Human peroxisomal enoyl-CoA hydratase 1 ELISA Kit;
  • Catalog
    E013204
  • species
    Human
  • GeneECH1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human ECH1. No significant cross-reactivity or interference between human ECH1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human ECH1 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
  • Intended UseHuman ECH1 ELISA Kit allows for the in vitro quantitative determination of ECH1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ECH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ECH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ECH1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ECH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial Protein information
  • Uniprot ID ECH1_HUMAN
  • Uniprot AC DIRYCAQD AFFQVKEVDV GLAADVGTLQ RLPKVIGNQS LVNELAFTAR KMMADEALGS
  • UniGene Hs.196176;
  • GeneID 1891
  • KEGG hsa:1891;
  • Human Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial Protein SEQUENCE

  • SEQUENCE 328 AA; 35816 MW; 211E0FF40379E6DA CRC64;

    MAAGIVASRR LRDLLTRRLT GSNYPGLSIS LRLTGSSAQE EASGVALGEA

    PDHSYESLRV TSAQKHVLHV QLNRPNKRNA MNKVFWREMV ECFNKISRDA

    DCRAVVISGA GKMFTAGIDL MDMASDILQP KGDDVARISW YLRDIITRYQ

    ETFNVIERCP KPVIAAVHGG CIGGGVDLVT ACDIRYCAQD AFFQVKEVDV

    GLAADVGTLQ RLPKVIGNQS LVNELAFTAR KMMADEALGS GLVSRVFPDK

    EVMLDAALAL AAEISSKSPV AVQSTKVNLL YSRDHSVAES LNYVASWNMS

    MLQTQDLVKS VQATTENKEL KTVTFSKL

  • UCSC uc002oji.4; human.;



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