Human EFNB2 ELISA Kit

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  • Alternative name

    Human eph-related receptor tyrosine kinase ligand 5 ELISA Kit;Human LERK-5 ELISA Kit;Human HTK ligand ELISA Kit;Human Htk-L ELISA Kit;Human EPLG5 ELISA Kit;Human HTKL ELISA Kit;Human LERK5 ELISA Kit;Human ephrin B2 ELISA Kit;Human ephrin-B2 ELISA Kit;Human ligand of eph-related kinase 5 ELISA Kit;

  • Catalog
    E013326
  • species
    Human
  • GeneEFNB2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Ephrin B2 (EFNB2). No significant cross-reactivity or interference between Ephrin B2 (EFNB2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.059ng/mL.
  • Intended UseHuman EFNB2 ELISA Kit allows for the in vitro quantitative determination of EFNB2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ephrin B2 (EFNB2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ephrin B2 (EFNB2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ephrin B2 (EFNB2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ephrin B2 (EFNB2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Ephrin-B2 Protein information
  • Uniprot ID EFNB2_HUMAN
  • Uniprot AC P52799; Q5JV56;
  • UniGene Hs.149239;
  • GeneID 1948
  • KEGG hsa:1948;
  • Human Ephrin-B2 Protein SEQUENCE
  • SEQUENCE 333 AA; 36923 MW; 6D9932A632626AEA CRC64;

    MAVRRDSVWK YCWGVLMVLC RTAISKSIVL EPIYWNSSNS KFLPGQGLVL

    YPQIGDKLDI ICPKVDSKTV GQYEYYKVYM VDKDQADRCT IKKENTPLLN

    CAKPDQDIKF TIKFQEFSPN LWGLEFQKNK DYYIISTSNG SLEGLDNQEG

    GVCQTRAMKI LMKVGQDASS AGSTRNKDPT RRPELEAGTN GRSSTTSPFV

    KPNPGSSTDG NSAGHSGNNI LGSEVALFAG IASGCIIFIV IIITLVVLLL

    KYRRRHRKHS PQHTTTLSLS TLATPKRSGN NNGSEPSDII IPLRTADSVF

    CPHYEKVSGD YGHPVYIVQE MPPQSPANIY YKV

  • UCSC uc001vqi.4; human.;



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