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Alternative name
Ephrin type-B receptor 2 ELISA KIT; Developmentally-regulated Eph-related tyrosine kinase ELISA KIT; ELK-related tyrosine kinase ELISA KIT; EPH tyrosine kinase 3 ELISA KIT; EPH-like kinase 5 ELISA KIT; EK5 ELISA KIT; hEK5 ELISA KIT; Renal carcinoma antigen NY-REN-47 ELISA KIT; Tyrosine-protein kinase TYRO5 ELISA KIT; Tyrosine-protein kinase receptor EPH-3EPHB2 ELISA KIT; DRT ELISA KIT; EPHT3 ELISA KIT; EPTH3 ELISA KIT; ERK ELISA KIT; HEK5 ELISA KIT; TYRO5 ELISA KIT; EK5 ELISA KIT; hEK5 ELISA KIT
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Catalog
E013546
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species
Human
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GeneERK
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Human ERK. No significant cross-reactivity or interference between Human ERK and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human ERK and all the analogues, therefore, cross reaction may still exist.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseHuman ERK ELISA Kit allows for the in vitro quantitative determination of ERK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ERK in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ERK. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human ERK and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ERK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human ERK. You can calculate the concentration of Human ERK in the samples by comparing the OD of the samples to the standard curve.
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