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Human EBAG9 ELISA Kit

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  • Alternative name

    Human cancer-associated surface antigen RCAS1 ELISA Kit;Human estrogen receptor-binding fragment-associated gene 9 protein ELISA Kit;Human RCAS1 ELISA Kit;Human EB9 ELISA Kit;Human PDAF ELISA Kit;Human estrogen receptor binding site associated, antigen, 9 ELISA Kit;Human receptor-binding cancer antigen expressed on SiSo cells ELISA Kit;
  • Catalog
    E013654
  • species
    Human
  • GeneEBAG9
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human EBAG9. No significant cross-reactivity or interference between Human EBAG9 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.195 U/ml
  • Intended UseHuman EBAG9 ELISA Kit allows for the in vitro quantitative determination of EBAG9 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Introduction: Receptor-binding cancer antigen expressed on SiSo cells (RCAS1) is a human tumor-associated antigen that induces cell cycle arrest and/or apoptosis in RCAS1 receptor-expressing immune cells. RCAS1 has been identified by means of a monoclonal antibody on the human uterine cervical adenocarcinoma cell line SiSo. RCAS1 is identical with the human estrogen-responsive gene product EBAG-9 (ER-binding fragment-associated antigen-9). RCAS1 is expressed at a high frequency in human uterine and ovarian cancer cells as well as in other mammalian cancer cells. High expression of RCAS1 has been shown to correlate significantly with tumour progression and predicted poor outcome in gallbladder cancer. RCAS1 is detected in nearly 100 % of gastric carcinomas and is strictly associated with tumor progression of gastric cancer. RCAS1 is highly expressed also in extramammary Paget's disease and in advanced stage squamous cell carcinoma of the skin. Ohshima et al have reported that RCAS1 is expressed in Reed-Sternberg cells in association with Epstein-Barr virus infection and that RCAS1 rather than FAS ligand allows these cells to evade host immune responses. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RCAS1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for RCAS1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain RCAS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of RCAS1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Receptor-binding cancer antigen expressed on SiSo cells Protein information
  • Uniprot ID RCAS1_HUMAN
  • Uniprot AC O00559; A8K3N6; Q5Y8C7; Q6IB20; Q9BS76;
  • UniGene Hs.409368; Hs.632960;
  • GeneID 9166
  • KEGG hsa:9166;
  • Human Receptor-binding cancer antigen expressed on SiSo cells Protein SEQUENCE

  • SEQUENCE 213 AA; 24377 MW; B115E741E23891C5 CRC64;

    MAITQFRLFK FCTCLATVFS FLKRLICRSG RGRKLSGDQI TLPTTVDYSS

    VPKQTDVEEW TSWDEDAPTS VKIEGGNGNV ATQQNSLEQL EPDYFKDMTP

    TIRKTQKIVI KKREPLNFGI PDGSTGFSSR LAATQDLPFI HQSSELGDLD

    TWQENTNAWE EEEDAAWQAE EVLRQQKLAD REKRAAEQQR KKMEKEAQRL

    MKKEQNKIGV KLS

  • UCSC uc003ynf.5; human. [O00559-1];



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