Human FCER1A ELISA Kit

Alternative name
Human Fc-epsilon RI-alpha ELISA Kit;Human FcERI ELISA Kit;Human igE Fc receptor subunit alpha ELISA Kit;Human FCE1A ELISA Kit;Human Fc fragment of IgE receptor Ia ELISA Kit;Human high affinity immunoglobulin epsilon receptor subunit alpha ELISA Kit;Human Fc IgE receptor, alpha polypeptide ELISA Kit;Human Fc epsilon RI alpha-chain ELISA Kit;Human Fc epsilon receptor Ia ELISA Kit;Human Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide ELISA Kit;Human high affinity immunoglobulin epsilon receptor alpha-subunit ELISA Kit;Human immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide ELISA Kit;
Catalog
E014589
species
Human
GeneFCER1A
SpecificityThis assay has high sensitivity and excellent specificity for detection of human FCER1A. No significant cross-reactivity or interference between human FCER1A and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human FCER1A is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m
Intended UseHuman FCER1A ELISA Kit allows for the in vitro quantitative determination of FCER1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrincple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FCER1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FCER1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FCER1A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FCER1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human High affinity immunoglobulin epsilon receptor subunit alpha Protein information
Uniprot ID FCERA_HUMAN
Uniprot AC P12319;
UniGene Hs.897;
GeneID 2205
KEGG hsa:2205;
Human High affinity immunoglobulin epsilon receptor subunit alpha Protein SEQUENCE
SEQUENCE 257 AA; 29596 MW; F183BB2357DDAD58 CRC64;
MAPAMESPTL LCVALLFFAP DGVLAVPQKP KVSLNPPWNR IFKGENVTLT
CNGNNFFEVS STKWFHNGSL SEETNSSLNI VNAKFEDSGE YKCQHQQVNE
SEPVYLEVFS DWLLLQASAE VVMEGQPLFL RCHGWRNWDV YKVIYYKDGE
ALKYWYENHN ISITNATVED SGTYYCTGKV WQLDYESEPL NITVIKAPRE
KYWLQFFIPL LVVILFAVDT GLFISTQQQV TFLLKIKRTR KGFRLLNPHP
KPNPKNN
UCSC uc001ftq.4; human.;

Category

Human FCER1A ELISA Kit