Human FGF21 ELISA Kit

Alternative name
Human FGF-21 ELISA Kit;Human fibroblast growth factor 21 ELISA Kit;
Catalog
E014768
species
Human
GeneFGF21
SpecificityNatural and recombinant human FGF21
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman FGF21 ELISA Kit allows for the in vitro quantitative determination of FGF21 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human FGF21 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FGF21 has been precoated onto 96-well plates. Standards(E.coli, H29-S209) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF21 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FGF21 amount of sample captured in plate. Background: Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene. By genomic sequence analysis, Nishimura et al. (2000) identified the FGF21 gene within the 5-prime flanking region of the FUT1 gene on chromosome 19. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 injection in ob/ob mice results in an increase in Glut1 in adipose tissue. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents.
Human Fibroblast growth factor 21 Protein information
Uniprot ID FGF21_HUMAN
Uniprot AC Q9NSA1; Q8N683;
UniGene Hs.283015;
GeneID 26291
KEGG hsa:26291;
Human Fibroblast growth factor 21 Protein SEQUENCE
SEQUENCE 209 AA; 22300 MW; 27925C52A0023823 CRC64;
MDSDETGFEH SGLWVSVLAG LLLGACQAHP IPDSSPLLQF GGQVRQRYLY
TDDAQQTEAH LEIREDGTVG GAADQSPESL LQLKALKPGV IQILGVKTSR
FLCQRPDGAL YGSLHFDPEA CSFRELLLED GYNVYQSEAH GLPLHLPGNK
SPHRDPAPRG PARFLPLPGL PPALPEPPGI LAPQPPDVGS SDPLSMVGPS
QGRSPSYAS
UCSC uc002pkn.2; human.;

Category

Human FGF21 ELISA Kit

Human FGF-21 ELISA Kit;Human fibroblast growth factor 21 ELISA Kit;