Human FGF4 ELISA Kit

Alternative name
Human FGF-4 ELISA Kit;Human Heparin secretory-transforming protein 1 ELISA Kit;Human HST ELISA Kit;Human HST-1 ELISA Kit;Human HSTF-1 ELISA Kit;Human heparin-binding growth factor 4 ELISA Kit;Human HBGF-4 ELISA Kit;Human transforming protein KS3 ELISA Kit;Human HSTF1 ELISA Kit;Human KS3 ELISA Kit;Human K-FGF ELISA Kit;Human KFGF ELISA Kit;Human fibroblast growth factor 4 ELISA Kit;Human fibroblast growth factor 4 splice isoform ELISA Kit;Human heparin secretory transforming protein 1 ELISA Kit;Human human stomach cancer, transforming factor from FGF-related oncogene ELISA Kit;Human kaposi sarcoma oncogene ELISA Kit;Human oncogene HST ELISA Kit;
Catalog
E014773
species
Human
GeneFGF4
SpecificityNatural and recombinant human FGF4
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman FGF4 ELISA Kit allows for the in vitro quantitative determination of FGF4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human FGF4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FGF4 has been precoated onto 96-well plates. Standards(E.coli, A31-L206) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FGF4 amount of sample captured in plate. Background: Fibroblast growth factor 4 is a protein that in humans is encoded by the FGF4 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was identified by its oncogenic transforming activity. This gene and FGF3, another oncogenic growth factor, are located closely on chromosome 11. FGF4, a direct target of LEF1 and Wnt signaling, can rescue the arrest of tooth organogenesis in Lef1(-/-) mice.
Human Fibroblast growth factor 4 Protein information
Uniprot ID FGF4_HUMAN
Uniprot AC P08620; B7U994;
UniGene Hs.1755;
GeneID 2249
KEGG hsa:2249;
Human Fibroblast growth factor 4 Protein SEQUENCE
SEQUENCE 206 AA; 22048 MW; C7FD54A0272A1569 CRC64;
MSGPGTAAVA LLPAVLLALL APWAGRGGAA APTAPNGTLE AELERRWESL
VALSLARLPV AAQPKEAAVQ SGAGDYLLGI KRLRRLYCNV GIGFHLQALP
DGRIGGAHAD TRDSLLELSP VERGVVSIFG VASRFFVAMS SKGKLYGSPF
FTDECTFKEI LLPNNYNAYE SYKYPGMFIA LSKNGKTKKG NRVSPTMKVT
HFLPRL
UCSC uc001opg.2; human. [P08620-1];

Category

Human FGF4 ELISA Kit