Human FCN1 ELISA Kit

Alternative name
Human Collagen/fibrinogen domain-containing protein 1 ELISA Kit;Human ficolin-A ELISA Kit;Human ficolin-alpha ELISA Kit;Human M-ficolin ELISA Kit;Human FCNM ELISA Kit;Human ficolin 1 ELISA Kit;Human ficolin-1 ELISA Kit;Human ficolin (collagen/fibrinogen domain-containing) 1 ELISA Kit;
Catalog
E015125
species
Human
GeneFCN1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Ficolin 1 (FCN1). No significant cross-reactivity or interference between Ficolin 1 (FCN1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.055ng/mL.
Intended UseHuman FCN1 ELISA Kit allows for the in vitro quantitative determination of FCN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ficolin 1 (FCN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ficolin 1 (FCN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ficolin 1 (FCN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ficolin 1 (FCN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Ficolin-1 Protein information
Uniprot ID FCN1_HUMAN
Uniprot AC O00602; Q5VYV5; Q92596;
UniGene Hs.440898; Hs.638586;
GeneID 2219
KEGG hsa:2219;
Human Ficolin-1 Protein SEQUENCE
SEQUENCE 326 AA; 35078 MW; 184D24B371B251AB CRC64;
MELSGATMAR GLAVLLVLFL HIKNLPAQAA DTCPEVKVVG LEGSDKLTIL
RGCPGLPGAP GPKGEAGVIG ERGERGLPGA PGKAGPVGPK GDRGEKGMRG
EKGDAGQSQS CATGPRNCKD LLDRGYFLSG WHTIYLPDCR PLTVLCDMDT
DGGGWTVFQR RMDGSVDFYR DWAAYKQGFG SQLGEFWLGN DNIHALTAQG
SSELRVDLVD FEGNHQFAKY KSFKVADEAE KYKLVLGAFV GGSAGNSLTG
HNNNFFSTKD QDNDVSSSNC AEKFQGAWWY ADCHASNLNG LYLMGPHESY
ANGINWSAAK GYKYSYKVSE MKVRPA
UCSC uc004cfi.4; human.;

Category

Human FCN1 ELISA Kit

Human Collagen/fibrinogen domain-containing protein 1 ELISA Kit;Human ficolin-A ELISA Kit;Human ficolin-alpha ELISA Kit;Human M-ficolin ELISA Kit;Human FCNM ELISA Kit;Human ficolin 1 ELISA Kit;Human ficolin-1 ELISA Kit;Human ficolin (collagen/fibrinogen domain-containing) 1 ELISA Kit;