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Catalog
E015551
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species
Human
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GeneFree-T3
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Human fT3. No significant cross-reactivity or interference between Human fT3 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity1.2 pmol/L (mean of 6 independent assays)
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Intended UseHuman Free-T3 ELISA Kit allows for the in vitro quantitative determination of Free-T3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalIntended Uses: For the quantitative detection of Human Free Tri-iodothyronine Indes (fT3) concentration in serum, plasma, cell culture supernates and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific for fT3 has been pre-coated onto a microplate. Standards or samples and a biotin-conjugated antibody specific for fT3 are pipetted into the wells. A competitive inhibition reaction is launched between fT3 (Standards or samples) and biotin-conjugated fT3 with the pre-coated antibody specific for fT3. After washing, streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of fT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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