Human FUCA1 ELISA Kit

Alternative name
Human alpha-L-fucosidase I ELISA Kit;Human alpha-L-fucoside fucohydrolase 1 ELISA Kit;Human alpha-L-fucosidase 1 ELISA Kit;Human FUCA ELISA Kit;Human fucosidase, alpha-L- 1, tissue ELISA Kit;Human tissue alpha-L-fucosidase ELISA Kit;
Catalog
E015647
species
Human
GeneFUCA1
SpecificityThis assay has high sensitivity and excellent specificity for detection of AFU. No significant cross-reactivity or interference between AFU and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AFU and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman FUCA1 ELISA Kit allows for the in vitro quantitative determination of FUCA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: AFU ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AFU. Standards or samples are then added to the microtiter plate wells and AFU if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AFU present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AFU are added to each well to ?sandwich? the AFU immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AFU and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AFU concentration in each sample is interpolated from this standard curve.
Human Tissue alpha-L-fucosidase Protein information
Uniprot ID FUCO_HUMAN
Uniprot AC P04066; B2RBG3; Q14334; Q14335; Q3LID0; Q8NAC2;
UniGene Hs.370858;
GeneID 2517
KEGG hsa:2517;
Human Tissue alpha-L-fucosidase Protein SEQUENCE
SEQUENCE 466 AA; 53689 MW; D9A38ECC7BADCBBB CRC64;
MRAPGMRSRP AGPALLLLLL FLGAAESVRR AQPPRRYTPD WPSLDSRPLP
AWFDEAKFGV FIHWGVFSVP AWGSEWFWWH WQGEGRPQYQ RFMRDNYPPG
FSYADFGPQF TARFFHPEEW ADLFQAAGAK YVVLTTKHHE GFTNWPSPVS
WNWNSKDVGP HRDLVGELGT ALRKRNIRYG LYHSLLEWFH PLYLLDKKNG
FKTQHFVSAK TMPELYDLVN SYKPDLIWSD GEWECPDTYW NSTNFLSWLY
NDSPVKDEVV VNDRWGQNCS CHHGGYYNCE DKFKPQSLPD HKWEMCTSID
KFSWGYRRDM ALSDVTEESE IISELVQTVS LGGNYLLNIG PTKDGLIVPI
FQERLLAVGK WLSINGEAIY ASKPWRVQWE KNTTSVWYTS KGSAVYAIFL
HWPENGVLNL ESPITTSTTK ITMLGIQGDL KWSTDPDKGL FISLPQLPPS
AVPAEFAWTI KLTGVK
UCSC uc001bie.5; human.;

Category

Human FUCA1 ELISA Kit

Human alpha-L-fucosidase I ELISA Kit;Human alpha-L-fucoside fucohydrolase 1 ELISA Kit;Human alpha-L-fucosidase 1 ELISA Kit;Human FUCA ELISA Kit;Human fucosidase, alpha-L- 1, tissue ELISA Kit;Human tissue alpha-L-fucosidase ELISA Kit;