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Human GPER ELISA Kit

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  • Alternative name

    G-protein coupled estrogen receptor 1 ELISA KIT; Chemoattractant receptor-like 2 ELISA KIT; Flow-induced endothelial G-protein coupled receptor 1 ELISA KIT; FEG-1 ELISA KIT; G protein-coupled estrogen receptor 1 ELISA KIT; G-protein coupled receptor 30 ELISA KIT; GPCR-Br ELISA KIT; IL8-related receptor DRY12 ELISA KIT; Lymphocyte-derived G-protein coupled receptor ELISA KIT; LYGPR ELISA KIT; Membrane estrogen receptor ELISA KIT; mERGPER1 ELISA KIT; CEPR ELISA KIT; CMKRL2 ELISA KIT; DRY12 ELISA KIT; GPER ELISA KIT; GPR30 ELISA KIT; FEG-1 ELISA KIT; LYGPR ELISA KIT; mER ELISA KIT
  • Catalog
    E015766
  • species
    Human
  • GeneGPER
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of G Protein Coupled Estrogen Receptor 1 (GPER). No significant cross-reactivity or interference between G Protein Coupled Estrogen Receptor 1 (GPER) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.119ng/mL.
  • Intended UseHuman GPER ELISA Kit allows for the in vitro quantitative determination of GPER , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Coupled Estrogen Receptor 1 (GPER). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to G Protein Coupled Estrogen Receptor 1 (GPER). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain G Protein Coupled Estrogen Receptor 1 (GPER), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of G Protein Coupled Estrogen Receptor 1 (GPER) in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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