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Alternative name
Solute carrier family 52, riboflavin transporter, member 2 ELISA KIT; Porcine endogenous retrovirus A receptor 1 ELISA KIT; PERV-A receptor 1 ELISA KIT; Protein GPR172A ELISA KIT; Riboflavin transporter 3 ELISA KIT; hRFT3SLC52A2 ELISA KIT; GPR172A ELISA KIT; PAR1 ELISA KIT; RFT3 ELISA KIT; PERV-A receptor 1 ELISA KIT; hRFT3 ELISA KIT
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Catalog
E015775
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species
Human
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GeneGPR172A
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SpecificityThis assay has high sensitivity and excellent specificity for detection of G Protein Coupled Receptor 172A (GPR172A).
No significant cross-reactivity or interference between G Protein Coupled Receptor 172A (GPR172A) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.57ng/mL.
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Intended UseHuman GPR172A ELISA Kit allows for the in vitro quantitative determination of GPR172A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Coupled Receptor 172A (GPR172A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to G Protein Coupled Receptor 172A (GPR172A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain G Protein Coupled Receptor 172A (GPR172A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of G Protein Coupled Receptor 172A (GPR172A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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