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Alternative name
G-protein coupled receptor 124 ELISA KIT; Tumor endothelial marker 5GPR124 ELISA KIT; KIAA1531 ELISA KIT; TEM5 ELISA KIT
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Catalog
E015807
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species
Human
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GeneGPR124
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SpecificityThis assay has high sensitivity and excellent specificity for detection of human GPR124. No significant cross-reactivity or interference between human GPR124 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of human GPR124 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m
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Intended UseHuman GPR124 ELISA Kit allows for the in vitro quantitative determination of GPR124 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrincple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GPR124 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GPR124 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPR124 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPR124 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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