Human GRK5 ELISA Kit

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  • Alternative name

    Human g protein-coupled receptor kinase GRK5 ELISA Kit;Human GPRK5 ELISA Kit;Human G protein-coupled receptor kinase 5 ELISA Kit;Human FP2025 ELISA Kit;

  • Catalog
    E015848
  • species
    Human
  • GeneGRK5
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human GRK5. No significant cross-reactivity or interference between human GRK5 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 7 pg/ml
  • Intended UseHuman GRK5 ELISA Kit allows for the in vitro quantitative determination of GRK5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GRK5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GRK5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GRK5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GRK5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human G protein-coupled receptor kinase 5 Protein information
  • Uniprot ID GRK5_HUMAN
  • Uniprot AC P34947; D3DRD0; Q5T059;
  • UniGene Hs.524625; Hs.736460;
  • GeneID 2869
  • KEGG hsa:2869;
  • Human G protein-coupled receptor kinase 5 Protein SEQUENCE
  • SEQUENCE 590 AA; 67787 MW; D363567ECFF5CF21 CRC64;

    MELENIVANT VLLKAREGGG GKRKGKSKKW KEILKFPHIS QCEDLRRTID

    RDYCSLCDKQ PIGRLLFRQF CETRPGLECY IQFLDSVAEY EVTPDEKLGE

    KGKEIMTKYL TPKSPVFIAQ VGQDLVSQTE EKLLQKPCKE LFSACAQSVH

    EYLRGEPFHE YLDSMFFDRF LQWKWLERQP VTKNTFRQYR VLGKGGFGEV

    CACQVRATGK MYACKRLEKK RIKKRKGESM ALNEKQILEK VNSQFVVNLA

    YAYETKDALC LVLTIMNGGD LKFHIYNMGN PGFEEERALF YAAEILCGLE

    DLHRENTVYR DLKPENILLD DYGHIRISDL GLAVKIPEGD LIRGRVGTVG

    YMAPEVLNNQ RYGLSPDYWG LGCLIYEMIE GQSPFRGRKE KVKREEVDRR

    VLETEEVYSH KFSEEAKSIC KMLLTKDAKQ RLGCQEEGAA EVKRHPFFRN

    MNFKRLEAGM LDPPFVPDPR AVYCKDVLDI EQFSTVKGVN LDHTDDDFYS

    KFSTGSVSIP WQNEMIETEC FKELNVFGPN GTLPPDLNRN HPPEPPKKGL

    LQRLFKRQHQ NNSKSSPSSK TSFNHHINSN HVSSNSTGSS

  • UCSC uc001led.4; human.;



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