Human GLA ELISA Kit

Alternative name
Human alpha-D-galactosidase A ELISA Kit;Human Alpha-D-galactoside galactohydrolase ELISA Kit;Human melibiase ELISA Kit;Human GALA ELISA Kit;Human galactosidase alpha ELISA Kit;Human alpha-galactosidase A ELISA Kit;Human agalsidase alfa ELISA Kit;Human alpha-D-galactoside galactohydrolase 1 ELISA Kit;Human alpha-gal A ELISA Kit;
Catalog
E015935
species
Human
GeneGLA
SpecificityThis assay has high sensitivity and excellent specificity for detection of Galactosidase Alpha (GLa). No significant cross-reactivity or interference between Galactosidase Alpha (GLa) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.55ng/mL.
Intended UseHuman GLA ELISA Kit allows for the in vitro quantitative determination of GLA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galactosidase Alpha (GLa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Galactosidase Alpha (GLa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galactosidase Alpha (GLa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Galactosidase Alpha (GLa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Alpha-galactosidase A Protein information
Uniprot ID AGAL_HUMAN
Uniprot AC P06280; Q6LER7;
UniGene Hs.69089;
GeneID 2717
KEGG hsa:2717;
Human Alpha-galactosidase A Protein SEQUENCE
SEQUENCE 429 AA; 48767 MW; 613F8BF21B107D7B CRC64;
MQLRNPELHL GCALALRFLA LVSWDIPGAR ALDNGLARTP TMGWLHWERF
MCNLDCQEEP DSCISEKLFM EMAELMVSEG WKDAGYEYLC IDDCWMAPQR
DSEGRLQADP QRFPHGIRQL ANYVHSKGLK LGIYADVGNK TCAGFPGSFG
YYDIDAQTFA DWGVDLLKFD GCYCDSLENL ADGYKHMSLA LNRTGRSIVY
SCEWPLYMWP FQKPNYTEIR QYCNHWRNFA DIDDSWKSIK SILDWTSFNQ
ERIVDVAGPG GWNDPDMLVI GNFGLSWNQQ VTQMALWAIM AAPLFMSNDL
RHISPQAKAL LQDKDVIAIN QDPLGKQGYQ LRQGDNFEVW ERPLSGLAWA
VAMINRQEIG GPRSYTIAVA SLGKGVACNP ACFITQLLPV KRKLGFYEWT
SRLRSHINPT GTVLLQLENT MQMSLKDLL

Category

Human GLA ELISA Kit