Human GALR1 ELISA Kit

Alternative name
Human GAL1-R ELISA Kit;Human GALR-1 ELISA Kit;Human GALNR ELISA Kit;Human GALNR1 ELISA Kit;Human galanin receptor 1 ELISA Kit;Human galanin receptor type 1 ELISA Kit;
Catalog
E015959
species
Human
GeneGALR1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman GALR1 ELISA Kit allows for the in vitro quantitative determination of GALR1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This GALR1 ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of GALR1 in the sample, this GALR1 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus GALR1 concentration. The concentration of GALR1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: This GALR1 enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for GALR1. Standards or samples are then added to the microtiter plate wells and GALR1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GALR1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GALR1 are added to each well to "sandwich" the GALR1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain GALR1 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
Human Galanin receptor type 1 Protein information
Uniprot ID GALR1_HUMAN
Uniprot AC P47211; Q4VBL7;
UniGene Hs.272191;
GeneID 2587
KEGG hsa:2587;
Human Galanin receptor type 1 Protein SEQUENCE
SEQUENCE 349 AA; 38953 MW; A0664227157B3410 CRC64;
MELAVGNLSE GNASWPEPPA PEPGPLFGIG VENFVTLVVF GLIFALGVLG
NSLVITVLAR SKPGKPRSTT NLFILNLSIA DLAYLLFCIP FQATVYALPT
WVLGAFICKF IHYFFTVSML VSIFTLAAMS VDRYVAIVHS RRSSSLRVSR
NALLGVGCIW ALSIAMASPV AYHQGLFHPR ASNQTFCWEQ WPDPRHKKAY
VVCTFVFGYL LPLLLICFCY AKVLNHLHKK LKNMSKKSEA SKKKTAQTVL
VVVVVFGISW LPHHIIHLWA EFGVFPLTPA SFLFRITAHC LAYSNSSVNP
IIYAFLSENF RKAYKQVFKC HIRKDSHLSD TKESKSRIDT PPSTNCTHV
UCSC uc002lms.5; human.;

Category

Human GALR1 ELISA Kit

Human GAL1-R ELISA Kit;Human GALR-1 ELISA Kit;Human GALNR ELISA Kit;Human GALNR1 ELISA Kit;Human galanin receptor 1 ELISA Kit;Human galanin receptor type 1 ELISA Kit;