Human GJB1 ELISA Kit

Alternative name
Human connexin-32 ELISA Kit;Human CX32 ELISA Kit;Human GAP junction 28 kDa liver protein ELISA Kit;Human CMTX ELISA Kit;Human CMTX1 ELISA Kit;Human gap junction protein beta 1 ELISA Kit;Human gap junction beta-1 protein ELISA Kit;Human gap junction protein, beta 1, 32kDa ELISA Kit;
Catalog
E016146
species
Human
GeneGJB1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Gap Junction Protein Beta 1 (GJb1). No significant cross-reactivity or interference between Gap Junction Protein Beta 1 (GJb1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.108ng/mL.
Intended UseHuman GJB1 ELISA Kit allows for the in vitro quantitative determination of GJB1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gap Junction Protein Beta 1 (GJb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gap Junction Protein Beta 1 (GJb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gap Junction Protein Beta 1 (GJb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gap Junction Protein Beta 1 (GJb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Gap junction beta-1 protein Protein information
Uniprot ID CXB1_HUMAN
Uniprot AC P08034; B2R8R2; D3DVV2; Q5U0S4;
UniGene Hs.333303;
GeneID 2705
KEGG hsa:2705;
Human Gap junction beta-1 protein Protein SEQUENCE
SEQUENCE 283 AA; 32025 MW; 8222C4811D12451E CRC64;
MNWTGLYTLL SGVNRHSTAI GRVWLSVIFI FRIMVLVVAA ESVWGDEKSS
FICNTLQPGC NSVCYDQFFP ISHVRLWSLQ LILVSTPALL VAMHVAHQQH
IEKKMLRLEG HGDPLHLEEV KRHKVHISGT LWWTYVISVV FRLLFEAVFM
YVFYLLYPGY AMVRLVKCDV YPCPNTVDCF VSRPTEKTVF TVFMLAASGI
CIILNVAEVV YLIIRACARR AQRRSNPPSR KGSGFGHRLS PEYKQNEINK
LLSEQDGSLK DILRRSPGTG AGLAEKSDRC SAC
UCSC uc004dzf.4; human.;

Category

Human GJB1 ELISA Kit

Human connexin-32 ELISA Kit;Human CX32 ELISA Kit;Human GAP junction 28 kDa liver protein ELISA Kit;Human CMTX ELISA Kit;Human CMTX1 ELISA Kit;Human gap junction protein beta 1 ELISA Kit;Human gap junction beta-1 protein ELISA Kit;Human gap junction protein, beta 1, 32kDa ELISA Kit;