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Alternative name
Gastrin ELISA KIT; Gastrin component IGAST ELISA KIT; GAS ELISA KIT; G52 ELISA KIT; G34 ELISA KIT; G17 ELISA KIT; G14 ELISA KIT; G6 ELISA KIT
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Catalog
E016205
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species
Human
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GeneGT
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SpecificityThis assay has high sensitivity and excellent specificity for detection of GT. No significant cross-reactivity or interference between GT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GT and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity1.0 pg/mL
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Intended UseHuman GT ELISA Kit allows for the in vitro quantitative determination of GT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyCardiovascular
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Product Description
specificalPrinciple of the Assay: GT ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GT. Standards or samples are then added to the microtiter plate wells and GT if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GT present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GT are added to each well to "sandwich" the GT immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GT and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GT concentration in each sample is interpolated from this standard curve.
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