SpecificityThis assay has high sensitivity and excellent specificity for detection of Gastrin Releasing Peptide (GRP).
No significant cross-reactivity or interference between Gastrin Releasing Peptide (GRP) and analogues was observed.
Intended UseHuman GRP ELISA Kit allows for the in vitro quantitative determination of GRP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Gastrin Releasing Peptide (GRP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Gastrin Releasing Peptide (GRP) and unlabeled Gastrin Releasing Peptide (GRP) (Standards or samples) with the pre-coated antibody specific to Gastrin Releasing Peptide (GRP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Gastrin Releasing Peptide (GRP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Gastrin Releasing Peptide (GRP) in the sample.
Human Neuromedin-C Protein information
Uniprot ID
GRP_HUMAN
Uniprot AC
P07492; P07491; P81553; Q14454; Q53YA0; Q9BSY7;