Human GATA4 ELISA Kit

Alternative name
Human GATA-binding factor 4 ELISA Kit;Human ASD2 ELISA Kit;Human TACHD ELISA Kit;Human TOF ELISA Kit;Human VSD1 ELISA Kit;Human GATA binding protein 4 ELISA Kit;Human transcription factor GATA-4 ELISA Kit;
Catalog
E016256
species
Human
GeneGATA4
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman GATA4 ELISA Kit allows for the in vitro quantitative determination of GATA4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalFor Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma Intended Uses: This GATA4 ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of GATA4 in the sample, this GATA4 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus GATA4 concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of GATA4 utilizes a multiclonal anti-GATA4 antibody and an GATA4-HRP conjugate. The assay sample and buffer are incubated together with GATA4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GATA4 concentration since GATA4 from samples and GATA4-HRP conjugate compete for the anti-GATA4 antibody binding site. Since the number of sites is limited, as more sites are occupied by GATA4 from the sample, fewer sites are left to bind GATA4-HRP conjugate. Standards of known GATA4 concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of GATA4. The GATA4 concentration in each sample is interpolated from this standard curve.
Human Transcription factor GATA-4 Protein information
Uniprot ID GATA4_HUMAN
Uniprot AC P43694; B7ZKX0; B7ZKZ4; Q3MJ45; Q5IFM8;
UniGene Hs.243987;
GeneID 2626
KEGG hsa:2626;
Human Transcription factor GATA-4 Protein SEQUENCE
SEQUENCE 442 AA; 44565 MW; 141B8CD841E12C7B CRC64;
MYQSLAMAAN HGPPPGAYEA GGPGAFMHGA GAASSPVYVP TPRVPSSVLG
LSYLQGGGAG SASGGASGGS SGGAASGAGP GTQQGSPGWS QAGADGAAYT
PPPVSPRFSF PGTTGSLAAA AAAAAAREAA AYSSGGGAAG AGLAGREQYG
RAGFAGSYSS PYPAYMADVG ASWAAAAAAS AGPFDSPVLH SLPGRANPAA
RHPNLDMFDD FSEGRECVNC GAMSTPLWRR DGTGHYLCNA CGLYHKMNGI
NRPLIKPQRR LSASRRVGLS CANCQTTTTT LWRRNAEGEP VCNACGLYMK
LHGVPRPLAM RKEGIQTRKR KPKNLNKSKT PAAPSGSESL PPASGASSNS
SNATTSSSEE MRPIKTEPGL SSHYGHSSSV SQTFSVSAMS GHGPSIHPVL
SALKLSPQGY ASPVSQSPQT SSKQDSWNSL VLADSHGDII TA
UCSC uc003wuc.3; human. [P43694-1];

Category

Human GATA4 ELISA Kit

Human GATA-binding factor 4 ELISA Kit;Human ASD2 ELISA Kit;Human TACHD ELISA Kit;Human TOF ELISA Kit;Human VSD1 ELISA Kit;Human GATA binding protein 4 ELISA Kit;Human transcription factor GATA-4 ELISA Kit;