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Alternative name
Ghrelin O-acyltransferase ELISA KIT; Membrane-bound O-acyltransferase domain-containing protein 4 ELISA KIT; O-acyltransferase domain-containing protein 4MBOAT4 ELISA KIT; GOAT ELISA KIT; OACT4 ELISA KIT; O-acyltransferase domain-containing protein 4 ELISA KIT
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Catalog
E016406
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species
Human
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GeneGOAT
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SpecificityThis assay has high sensitivity and excellent specificity for detection of GOAT. No significant cross-reactivity or interference between GOAT and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.29ng/mL.
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Intended UseHuman GOAT ELISA Kit allows for the in vitro quantitative determination of GOAT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalIntended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GOAT in human serum, plasma, tissue homogenates and other biological fluids.
Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to GOAT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GOAT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GOAT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GOAT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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