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Catalog
E016529
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species
Human
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GeneGLP2
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Glucagon Like Peptide 2 (GLP2).
No significant cross-reactivity or interference between Glucagon Like Peptide 2 (GLP2) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 44.3pg/mL.
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Intended UseHuman GLP2 ELISA Kit allows for the in vitro quantitative determination of GLP2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Glucagon Like Peptide 2 (GLP2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Glucagon Like Peptide 2 (GLP2) and unlabeled Glucagon Like Peptide 2 (GLP2) (Standards or samples) with the pre-coated antibody specific to Glucagon Like Peptide 2 (GLP2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Glucagon Like Peptide 2 (GLP2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Glucagon Like Peptide 2 (GLP2) in the sample.
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