Human GPI ELISA Kit

Alternative name
Human GPI ELISA Kit;Human autocrine motility factor ELISA Kit;Human AMF ELISA Kit;Human neuroleukin ELISA Kit;Human NLK ELISA Kit;Human phosphoglucose isomerase ELISA Kit;Human PGI ELISA Kit;Human phosphohexose isomerase ELISA Kit;Human PHI ELISA Kit;Human Sperm antigen 36 ELISA Kit;Human SA-36 ELISA Kit;Human GNPI ELISA Kit;Human SA36 ELISA Kit;Human glucose-6-phosphate isomerase ELISA Kit;Human hexose monophosphate isomerase ELISA Kit;Human hexosephosphate isomerase ELISA Kit;Human oxoisomerase ELISA Kit;Human phosphohexomutase ELISA Kit;Human phosphosaccharomutase ELISA Kit;Human sperm antigen-36 ELISA Kit;
Catalog
E016601
species
Human
GeneGPI
SpecificityThis assay has high sensitivity and excellent specificity for detection of Glucose 6 Phosphate Isomerase (GPI). No significant cross-reactivity or interference between Glucose 6 Phosphate Isomerase (GPI) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.059ng/mL.
Intended UseHuman GPI ELISA Kit allows for the in vitro quantitative determination of GPI , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose 6 Phosphate Isomerase (GPI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucose 6 Phosphate Isomerase (GPI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose 6 Phosphate Isomerase (GPI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucose 6 Phosphate Isomerase (GPI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Glucose-6-phosphate isomerase Protein information
Uniprot ID G6PI_HUMAN
Uniprot AC P06744; B4DG39; Q9BRD3; Q9BSK5; Q9UHE6;
UniGene Hs.466471; Hs.515344;
GeneID 2821
KEGG hsa:2821;
Human Glucose-6-phosphate isomerase Protein SEQUENCE
SEQUENCE 558 AA; 63147 MW; 7C8E95277BDC79A6 CRC64;
MAALTRDPQF QKLQQWYREH RSELNLRRLF DANKDRFNHF SLTLNTNHGH
ILVDYSKNLV TEDVMRMLVD LAKSRGVEAA RERMFNGEKI NYTEGRAVLH
VALRNRSNTP ILVDGKDVMP EVNKVLDKMK SFCQRVRSGD WKGYTGKTIT
DVINIGIGGS DLGPLMVTEA LKPYSSGGPR VWYVSNIDGT HIAKTLAQLN
PESSLFIIAS KTFTTQETIT NAETAKEWFL QAAKDPSAVA KHFVALSTNT
TKVKEFGIDP QNMFEFWDWV GGRYSLWSAI GLSIALHVGF DNFEQLLSGA
HWMDQHFRTT PLEKNAPVLL ALLGIWYINC FGCETHAMLP YDQYLHRFAA
YFQQGDMESN GKYITKSGTR VDHQTGPIVW GEPGTNGQHA FYQLIHQGTK
MIPCDFLIPV QTQHPIRKGL HHKILLANFL AQTEALMRGK STEEARKELQ
AAGKSPEDLE RLLPHKVFEG NRPTNSIVFT KLTPFMLGAL VAMYEHKIFV
QGIIWDINSF DQWGVELGKQ LAKKIEPELD GSAQVTSHDA STNGLINFIK
QQREARVQ
UCSC uc002nvg.3; human. [P06744-1];

Category

Human GPI ELISA Kit