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Human GLUT3 ELISA Kit

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  • Alternative name

    Solute carrier family 2, facilitated glucose transporter member 3 ELISA KIT; Glucose transporter type 3, brain ELISA KIT; GLUT-3SLC2A3 ELISA KIT; GLUT3 ELISA KIT; GLUT-3 ELISA KIT
  • Catalog
    E016627
  • species
    Human
  • GeneGLUT3
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman GLUT3 ELISA Kit allows for the in vitro quantitative determination of GLUT3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Principle of the Assay: GLUT3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GLUT3. Standards or samples are then added to the microtiter plate wells and GLUT3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GLUT3 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for GLUT3 are added to each well to "sandwich" the GLUT3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GLUT3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GLUT3 concentration in each sample is interpolated from this standard curve.



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