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Human G6PC ELISA Kit

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  • Alternative name

    Human G-6-Pase ELISA Kit;Human G6Pase ELISA Kit;Human glucose-6-phosphatase alpha ELISA Kit;Human G6Pase-alpha ELISA Kit;Human G6PT ELISA Kit;Human G6PC1 ELISA Kit;Human GSD1 ELISA Kit;Human GSD1a ELISA Kit;Human glucose-6-phosphatase catalytic subunit ELISA Kit;Human glucose-6-phosphatase ELISA Kit;
  • Catalog
    E016646
  • species
    Human
  • GeneG6PC
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Glucose-6-Phosphatase, Catalytic (G6PC). No significant cross-reactivity or interference between Glucose-6-Phosphatase, Catalytic (G6PC) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.063ng/mL.
  • Intended UseHuman G6PC ELISA Kit allows for the in vitro quantitative determination of G6PC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose-6-Phosphatase, Catalytic (G6PC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucose-6-Phosphatase, Catalytic (G6PC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose-6-Phosphatase, Catalytic (G6PC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucose-6-Phosphatase, Catalytic (G6PC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Glucose-6-phosphatase Protein information
  • Uniprot ID G6PC_HUMAN
  • Uniprot AC P35575; A1L4C0; B4E1C3; K7EL82;
  • UniGene Hs.212293; Hs.742566;
  • GeneID 2538
  • KEGG hsa:2538;
  • Human Glucose-6-phosphatase Protein SEQUENCE

  • SEQUENCE 357 AA; 40484 MW; 2FEA1C78928A9919 CRC64;

    MEEGMNVLHD FGIQSTHYLQ VNYQDSQDWF ILVSVIADLR NAFYVLFPIW

    FHLQEAVGIK LLWVAVIGDW LNLVFKWILF GQRPYWWVLD TDYYSNTSVP

    LIKQFPVTCE TGPGSPSGHA MGTAGVYYVM VTSTLSIFQG KIKPTYRFRC

    LNVILWLGFW AVQLNVCLSR IYLAAHFPHQ VVAGVLSGIA VAETFSHIHS

    IYNASLKKYF LITFFLFSFA IGFYLLLKGL GVDLLWTLEK AQRWCEQPEW

    VHIDTTPFAS LLKNLGTLFG LGLALNSSMY RESCKGKLSK WLPFRLSSIV

    ASLVLLHVFD SLKPPSQVEL VFYVLSFCKS AVVPLASVSV IPYCLAQVLG

    QPHKKSL

  • UCSC uc002icb.3; human. [P35575-1];



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