Human GBA ELISA Kit

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  • Alternative name

    Human acid beta-glucosidase ELISA Kit;Human alglucerase ELISA Kit;Human beta-glucocerebrosidase ELISA Kit;Human beta-GC ELISA Kit;Human D-glucosyl-N-acylsphingosine glucohydrolase ELISA Kit;Human imiglucerase ELISA Kit;Human GC ELISA Kit;Human GLUC ELISA Kit;Human GBA1 ELISA Kit;Human GCB ELISA Kit;Human glucosylceramidase beta ELISA Kit;Human glucosylceramidase ELISA Kit;Human glucosidase, beta, acid ELISA Kit;Human glucosylceramidase-like protein ELISA Kit;Human lysosomal glucocerebrosidase ELISA Kit;

  • Catalog
    E016671
  • species
    Human
  • GeneGBA
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human GBA. No significant cross-reactivity or interference between human GBA and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human GBA is typically less than 0.078 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
  • Intended UseHuman GBA ELISA Kit allows for the in vitro quantitative determination of GBA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GBA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GBA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GBA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GBA bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Glucosylceramidase Protein information
  • Uniprot ID GLCM_HUMAN
  • Uniprot AC Q6I9R6; Q9UMJ8;
  • UniGene Hs.282997;
  • GeneID 2629
  • KEGG hsa:2629;
  • Human Glucosylceramidase Protein SEQUENCE
  • SEQUENCE 536 AA; 59716 MW; FA1E15684344A0E6 CRC64;

    MEFSSPSREE CPKPLSRVSI MAGSLTGLLL LQAVSWASGA RPCIPKSFGY

    SSVVCVCNAT YCDSFDPPTF PALGTFSRYE STRSGRRMEL SMGPIQANHT

    GTGLLLTLQP EQKFQKVKGF GGAMTDAAAL NILALSPPAQ NLLLKSYFSE

    EGIGYNIIRV PMASCDFSIR TYTYADTPDD FQLHNFSLPE EDTKLKIPLI

    HRALQLAQRP VSLLASPWTS PTWLKTNGAV NGKGSLKGQP GDIYHQTWAR

    YFVKFLDAYA EHKLQFWAVT AENEPSAGLL SGYPFQCLGF TPEHQRDFIA

    RDLGPTLANS THHNVRLLML DDQRLLLPHW AKVVLTDPEA AKYVHGIAVH

    WYLDFLAPAK ATLGETHRLF PNTMLFASEA CVGSKFWEQS VRLGSWDRGM

    QYSHSIITNL LYHVVGWTDW NLALNPEGGP NWVRNFVDSP IIVDITKDTF

    YKQPMFYHLG HFSKFIPEGS QRVGLVASQK NDLDAVALMH PDGSAVVVVL

    NRSSKDVPLT IKDPAVGFLE TISPGYSIHT YLWRRQ

  • UCSC uc001fjh.4; human. [P04062-1];



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