Human GAD2 ELISA Kit

Alternative name
Human 65 kDa glutamic acid decarboxylase ELISA Kit;Human GAD-65 ELISA Kit;Human glutamate decarboxylase 65 kDa isoform ELISA Kit;Human GAD65 ELISA Kit;Human glutamate decarboxylase 2 ELISA Kit;Human Glutamate decarboxylase-2 (pancreas) ELISA Kit;Human glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) ELISA Kit;
Catalog
E016709
species
Human
GeneGAD2
SpecificityThis assay has high sensitivity and excellent specificity for detection of human GAD65 . No significant cross-reactivity or interference between human GAD65 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human GAD65 and is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined t
Intended UseHuman GAD2 ELISA Kit allows for the in vitro quantitative determination of GAD2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrincple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GAD65 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GAD65 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GAD65 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GAD65 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Glutamate decarboxylase 2 Protein information
Uniprot ID DCE2_HUMAN
Uniprot AC Q05329; Q9UD87;
UniGene Hs.231829;
GeneID 2572
KEGG hsa:2572;
Human Glutamate decarboxylase 2 Protein SEQUENCE
SEQUENCE 585 AA; 65411 MW; 0322509F0E2C32EE CRC64;
MASPGSGFWS FGSEDGSGDS ENPGTARAWC QVAQKFTGGI GNKLCALLYG
DAEKPAESGG SQPPRAAARK AACACDQKPC SCSKVDVNYA FLHATDLLPA
CDGERPTLAF LQDVMNILLQ YVVKSFDRST KVIDFHYPNE LLQEYNWELA
DQPQNLEEIL MHCQTTLKYA IKTGHPRYFN QLSTGLDMVG LAADWLTSTA
NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS PGGAISNMYA
MMIARFKMFP EVKEKGMAAL PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI
LIKCDERGKM IPSDLERRIL EAKQKGFVPF LVSATAGTTV YGAFDPLLAV
ADICKKYKIW MHVDAAWGGG LLMSRKHKWK LSGVERANSV TWNPHKMMGV
PLQCSALLVR EEGLMQNCNQ MHASYLFQQD KHYDLSYDTG DKALQCGRHV
DVFKLWLMWR AKGTTGFEAH VDKCLELAEY LYNIIKNREG YEMVFDGKPQ
HTNVCFWYIP PSLRTLEDNE ERMSRLSKVA PVIKARMMEY GTTMVSYQPL
GDKVNFFRMV ISNPAATHQD IDFLIEEIER LGQDL

Category

Human GAD2 ELISA Kit