Human GPAM ELISA Kit

Alternative name
Human GPAT-1 ELISA Kit;Human GPAT1 ELISA Kit;Human KIAA1560 ELISA Kit;Human GPAT ELISA Kit;Human glycerol-3-phosphate acyltransferase, mitochondrial ELISA Kit;Human glycerol-3-phosphate acyltransferase 1, mitochondrial ELISA Kit;
Catalog
E016991
species
Human
GeneGPAM
SpecificityThis assay has high sensitivity and excellent specificity for detection of Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM). No significant cross-reactivity or interference between Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.55ng/mL.
Intended UseHuman GPAM ELISA Kit allows for the in vitro quantitative determination of GPAM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Glycerol-3-phosphate acyltransferase 1, mitochondrial Protein information
Uniprot ID GPAT1_HUMAN
Uniprot AC Q9HCL2; Q5VW51; Q86TA3;
UniGene Hs.42586;
GeneID 57678
KEGG hsa:57678;
Human Glycerol-3-phosphate acyltransferase 1, mitochondrial Protein SEQUENCE
SEQUENCE 828 AA; 93795 MW; 7AADD21BC8684693 CRC64;
MDESALTLGT IDVSYLPHSS EYSVGRCKHT SEEWGECGFR PTIFRSATLK
WKESLMSRKR PFVGRCCYSC TPQSWDKFFN PSIPSLGLRN VIYINETHTR
HRGWLARRLS YVLFIQERDV HKGMFATNVT ENVLNSSRVQ EAIAEVAAEL
NPDGSAQQQS KAVNKVKKKA KRILQEMVAT VSPAMIRLTG WVLLKLFNSF
FWNIQIHKGQ LEMVKAATET NLPLLFLPVH RSHIDYLLLT FILFCHNIKA
PYIASGNNLN IPIFSTLIHK LGGFFIRRRL DETPDGRKDV LYRALLHGHI
VELLRQQQFL EIFLEGTRSR SGKTSCARAG LLSVVVDTLS TNVIPDILII
PVGISYDRII EGHYNGEQLG KPKKNESLWS VARGVIRMLR KNYGCVRVDF
AQPFSLKEYL ESQSQKPVSA LLSLEQALLP AILPSRPSDA ADEGRDTSIN
ESRNATDESL RRRLIANLAE HILFTASKSC AIMSTHIVAC LLLYRHRQGI
DLSTLVEDFF VMKEEVLARD FDLGFSGNSE DVVMHAIQLL GNCVTITHTS
RNDEFFITPS TTVPSVFELN FYSNGVLHVF IMEAIIACSL YAVLNKRGLG
GPTSTPPNLI SQEQLVRKAA SLCYLLSNEG TISLPCQTFY QVCHETVGKF
IQYGILTVAE HDDQEDISPS LAEQQWDKKL PEPLSWRSDE EDEDSDFGEE
QRDCYLKVSQ SKEHQQFITF LQRLLGPLLE AYSSAAIFVH NFSGPVPEPE
YLQKLHKYLI TRTERNVAVY AESATYCLVK NAVKMFKDIG VFKETKQKRV
SVLELSSTFL PQCNRQKLLE YILSFVVL
UCSC uc001kzp.4; human.;

Category

Human GPAM ELISA Kit

Human GPAT-1 ELISA Kit;Human GPAT1 ELISA Kit;Human KIAA1560 ELISA Kit;Human GPAT ELISA Kit;Human glycerol-3-phosphate acyltransferase, mitochondrial ELISA Kit;Human glycerol-3-phosphate acyltransferase 1, mitochondrial ELISA Kit;