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Human AGL ELISA Kit

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  • Alternative name

    Human glycogen debrancher ELISA Kit;Human Oligo-1,4-1,4-glucantransferase ELISA Kit;Human Amylo-1,6-glucosidase ELISA Kit;Human Dextrin 6-alpha-D-glucosidase ELISA Kit;Human GDE ELISA Kit;Human amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase ELISA Kit;Human glycogen debranching enzyme ELISA Kit;Human amylo-1, 6-glucosidase, 4-alpha-glucanotransferase ELISA Kit;Human amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferaseprovided ELISA Kit;Human glycogen debranching protein ELISA Kit;
  • Catalog
    E017041
  • species
    Human
  • GeneAGL
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human AGL. No significant cross-reactivity or interference between Human AGL and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity15.6 pg/mL (mean of 6 independent assays).
  • Intended UseHuman AGL ELISA Kit allows for the in vitro quantitative determination of AGL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Intended Uses: For the quantitative detection of Human Glycogen debranching enzyme (AGL) concentration in serum, plasma and other biological fluids. Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitative AGL in Human serum, plasma. An antibody specific for AGL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AGL present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for AGL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGL bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Amylo-alpha-1,6-glucosidase Protein information
  • Uniprot ID GDE_HUMAN
  • Uniprot AC Q9UF08;
  • UniGene Hs.904;
  • GeneID 178
  • KEGG hsa:178;
  • Human Amylo-alpha-1,6-glucosidase Protein SEQUENCE

  • SEQUENCE 1532 AA; 174764 MW; 9BF1BCC43B7904D3 CRC64;

    MGHSKQIRIL LLNEMEKLEK TLFRLEQGYE LQFRLGPTLQ GKAVTVYTNY

    PFPGETFNRE KFRSLDWENP TEREDDSDKY CKLNLQQSGS FQYYFLQGNE

    KSGGGYIVVD PILRVGADNH VLPLDCVTLQ TFLAKCLGPF DEWESRLRVA

    KESGYNMIHF TPLQTLGLSR SCYSLANQLE LNPDFSRPNR KYTWNDVGQL

    VEKLKKEWNV ICITDVVYNH TAANSKWIQE HPECAYNLVN SPHLKPAWVL

    DRALWRFSCD VAEGKYKEKG IPALIENDHH MNSIRKIIWE DIFPKLKLWE

    FFQVDVNKAV EQFRRLLTQE NRRVTKSDPN QHLTIIQDPE YRRFGCTVDM

    NIALTTFIPH DKGPAAIEEC CNWFHKRMEE LNSEKHRLIN YHQEQAVNCL

    LGNVFYERLA GHGPKLGPVT RKHPLVTRYF TFPFEEIDFS MEESMIHLPN

    KACFLMAHNG WVMGDDPLRN FAEPGSEVYL RRELICWGDS VKLRYGNKPE

    DCPYLWAHMK KYTEITATYF QGVRLDNCHS TPLHVAEYML DAARNLQPNL

    YVVAELFTGS EDLDNVFVTR LGISSLIREA MSAYNSHEEG RLVYRYGGEP

    VGSFVQPCLR PLMPAIAHAL FMDITHDNEC PIVHRSAYDA LPSTTIVSMA

    CCASGSTRGY DELVPHQISV VSEERFYTKW NPEALPSNTG EVNFQSGIIA

    ARCAISKLHQ ELGAKGFIQV YVDQVDEDIV AVTRHSPSIH QSVVAVSRTA

    FRNPKTSFYS KEVPQMCIPG KIEEVVLEAR TIERNTKPYR KDENSINGTP

    DITVEIREHI QLNESKIVKQ AGVATKGPNE YIQEIEFENL SPGSVIIFRV

    SLDPHAQVAV GILRNHLTQF SPHFKSGSLA VDNADPILKI PFASLASRLT

    LAELNQILYR CESEEKEDGG GCYDIPNWSA LKYAGLQGLM SVLAEIRPKN

    DLGHPFCNNL RSGDWMIDYV SNRLISRSGT IAEVGKWLQA MFFYLKQIPR

    YLIPCYFDAI LIGAYTTLLD TAWKQMSSFV QNGSTFVKHL SLGSVQLCGV

    GKFPSLPILS PALMDVPYRL NEITKEKEQC CVSLAAGLPH FSSGIFRCWG

    RDTFIALRGI LLITGRYVEA RNIILAFAGT LRHGLIPNLL GEGIYARYNC

    RDAVWWWLQC IQDYCKMVPN GLDILKCPVS RMYPTDDSAP LPAGTLDQPL

    FEVIQEAMQK HMQGIQFRER NAGPQIDRNM KDEGFNITAG VDEETGFVYG

    GNRFNCGTWM DKMGESDRAR NRGIPATPRD GSAVEIVGLS KSAVRWLLEL

    SKKNIFPYHE VTVKRHGKAI KVSYDEWNRK IQDNFEKLFH VSEDPSDLNE

    KHPNLVHKRG IYKDSYGASS PWCDYQLRPN FTIAMVVAPE LFTTEKAWKA

    LEIAEKKLLG PLGMKTLDPD DMVYCGIYDN ALDNDNYNLA KGFNYHQGPE

    WLWPIGYFLR AKLYFSRLMG PETTAKTIVL VKNVLSRHYV HLERSPWKGL

    PELTNENAQY CPFSCETQAW SIATILETLY DL

  • UCSC uc001dsi.2; human. [P35573-1];



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