Human GSK3B ELISA Kit

Alternative name
Human GSK-3 beta ELISA Kit;Human serine/threonine-protein kinase GSK3B ELISA Kit;Human glycogen synthase kinase 3 beta ELISA Kit;Human glycogen synthase kinase-3 beta ELISA Kit;Human GSK3beta isoform ELISA Kit;
Catalog
E017087
species
Human
GeneGSK3B
SpecificityThis assay has high sensitivity and excellent specificity for detection of Glycogen Synthase Kinase 3 Beta (GSK3b). No significant cross-reactivity or interference between Glycogen Synthase Kinase 3 Beta (GSK3b) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.249ng/mL.
Intended UseHuman GSK3B ELISA Kit allows for the in vitro quantitative determination of GSK3B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycogen Synthase Kinase 3 Beta (GSK3b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycogen Synthase Kinase 3 Beta (GSK3b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycogen Synthase Kinase 3 Beta (GSK3b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycogen Synthase Kinase 3 Beta (GSK3b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Glycogen synthase kinase-3 beta Protein information
Uniprot ID GSK3B_HUMAN
Uniprot AC P49841; D3DN89; Q9BWH3; Q9UL47;
UniGene Hs.445733;
GeneID 2932
KEGG hsa:2932;
Human Glycogen synthase kinase-3 beta Protein SEQUENCE
SEQUENCE 420 AA; 46744 MW; 4ACC24D00CDBB9C3 CRC64;
MSGRPRTTSF AESCKPVQQP SAFGSMKVSR DKDGSKVTTV VATPGQGPDR
PQEVSYTDTK VIGNGSFGVV YQAKLCDSGE LVAIKKVLQD KRFKNRELQI
MRKLDHCNIV RLRYFFYSSG EKKDEVYLNL VLDYVPETVY RVARHYSRAK
QTLPVIYVKL YMYQLFRSLA YIHSFGICHR DIKPQNLLLD PDTAVLKLCD
FGSAKQLVRG EPNVSYICSR YYRAPELIFG ATDYTSSIDV WSAGCVLAEL
LLGQPIFPGD SGVDQLVEII KVLGTPTREQ IREMNPNYTE FKFPQIKAHP
WTKVFRPRTP PEAIALCSRL LEYTPTARLT PLEACAHSFF DELRDPNVKL
PNGRDTPALF NFTTQELSSN PPLATILIPP HARIQAAAST PTNATAASDA
NTGDRGQTNN AASASASNST
UCSC uc003edn.4; human. [P49841-1];

Category

Human GSK3B ELISA Kit

Human GSK-3 beta ELISA Kit;Human serine/threonine-protein kinase GSK3B ELISA Kit;Human glycogen synthase kinase 3 beta ELISA Kit;Human glycogen synthase kinase-3 beta ELISA Kit;Human GSK3beta isoform ELISA Kit;