Human GPC4 ELISA Kit

Alternative name
Human K-glypican ELISA Kit;Human glypican 4 ELISA Kit;Human glypican-4 ELISA Kit;Human dJ900E8.1 (glypican 4) ELISA Kit;Human glypican proteoglycan 4 ELISA Kit;
Catalog
E017222
species
Human
GeneGPC4
SpecificityThis assay has high sensitivity and excellent specificity for detection of human GPC4. No significant cross-reactivity or interference between human GPC4 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human GPC4 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman GPC4 ELISA Kit allows for the in vitro quantitative determination of GPC4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GPC4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GPC4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPC4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPC4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Secreted glypican-4 Protein information
Uniprot ID GPC4_HUMAN
Uniprot AC O75487; B2R6J7; B4E2C0; Q6ZMA6; Q96L43; Q9NU08; Q9UJN1; Q9UPD9;
UniGene Hs.58367;
GeneID 2239
KEGG hsa:2239;
Human Secreted glypican-4 Protein SEQUENCE
SEQUENCE 556 AA; 62412 MW; 827E07FAA0BD8188 CRC64;
MARFGLPALL CTLAVLSAAL LAAELKSKSC SEVRRLYVSK GFNKNDAPLH
EINGDHLKIC PQGSTCCSQE MEEKYSLQSK DDFKSVVSEQ CNHLQAVFAS
RYKKFDEFFK ELLENAEKSL NDMFVKTYGH LYMQNSELFK DLFVELKRYY
VVGNVNLEEM LNDFWARLLE RMFRLVNSQY HFTDEYLECV SKYTEQLKPF
GDVPRKLKLQ VTRAFVAART FAQGLAVAGD VVSKVSVVNP TAQCTHALLK
MIYCSHCRGL VTVKPCYNYC SNIMRGCLAN QGDLDFEWNN FIDAMLMVAE
RLEGPFNIES VMDPIDVKIS DAIMNMQDNS VQVSQKVFQG CGPPKPLPAG
RISRSISESA FSARFRPHHP EERPTTAAGT SLDRLVTDVK EKLKQAKKFW
SSLPSNVCND ERMAAGNGNE DDCWNGKGKS RYLFAVTGNG LANQGNNPEV
QVDTSKPDIL ILRQIMALRV MTSKMKNAYN GNDVDFFDIS DESSGEGSGS
GCEYQQCPSE FDYNATDHAG KSANEKADSA GVRPGAQAYL LTVFCILFLV
MQREWR
UCSC uc004exc.2; human. [O75487-1];

Category

Human GPC4 ELISA Kit

Human K-glypican ELISA Kit;Human glypican 4 ELISA Kit;Human glypican-4 ELISA Kit;Human dJ900E8.1 (glypican 4) ELISA Kit;Human glypican proteoglycan 4 ELISA Kit;