Human GPNMB ELISA Kit

Alternative name
Human transmembrane glycoprotein HGFIN ELISA Kit;Human HGFIN ELISA Kit;Human NMB ELISA Kit;Human glycoprotein nmb ELISA Kit;Human transmembrane glycoprotein NMB ELISA Kit;Human glycoprotein (transmembrane) nmb ELISA Kit;Human glycoprotein nmb-like protein ELISA Kit;Human glycoprotein nonmetastatic melanoma protein B ELISA Kit;Human hematopoietic growth factor inducible neurokinin-1 ELISA Kit;Human osteoactivin ELISA Kit;
Catalog
E017368
species
Human
GeneGPNMB
SpecificityNatural and recombinant human GPNMB
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman GPNMB ELISA Kit allows for the in vitro quantitative determination of GPNMB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human GPNMB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GPNMB has been precoated onto 96-well plates. Standards(NSO, A22-P486) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GPNMB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GPNMB amount of sample captured in plate. Background: Transmembrane glycoprotein NMB is a protein that in humans is encoded by the GPNMB gene. In osteoblast progenitor cells, GPNMB works as a positive regulator of osteoblast differentiation during later stages of matrix maturation and mineralization that is mediated at least in part by BMP-2 in a SMAD1 dependent manner to promote osteoblast differentiation. GPNMB can enhance the repairing process in bone fracture, demonstrated by its high expression during chondrogenesis (soft callus) and osteogenesis (hard callus) compared to the intact femurs that is why Osteoactivin (OA) could be a novel therapeutic agent used to treat generalized osteoporosis or localized osteopenia during fracture repair by stimulating bone growth and regeneration. Similarly, GPNMB expression increases during osteoclast differentiation and it is functionally implicated in this process, possibly by promoting the fusion of osteoclast progenitor cells.
Human Transmembrane glycoprotein NMB Protein information
Uniprot ID GPNMB_HUMAN
Uniprot AC Q14956; A4D155; Q6UVX1; Q8N1A1;
UniGene Hs.190495;
GeneID 10457
KEGG hsa:10457;
Human Transmembrane glycoprotein NMB Protein SEQUENCE
SEQUENCE 572 AA; 63923 MW; 2465C12C2F0F3996 CRC64;
MECLYYFLGF LLLAARLPLD AAKRFHDVLG NERPSAYMRE HNQLNGWSSD
ENDWNEKLYP VWKRGDMRWK NSWKGGRVQA VLTSDSPALV GSNITFAVNL
IFPRCQKEDA NGNIVYEKNC RNEAGLSADP YVYNWTAWSE DSDGENGTGQ
SHHNVFPDGK PFPHHPGWRR WNFIYVFHTL GQYFQKLGRC SVRVSVNTAN
VTLGPQLMEV TVYRRHGRAY VPIAQVKDVY VVTDQIPVFV TMFQKNDRNS
SDETFLKDLP IMFDVLIHDP SHFLNYSTIN YKWSFGDNTG LFVSTNHTVN
HTYVLNGTFS LNLTVKAAAP GPCPPPPPPP RPSKPTPSLA TTLKSYDSNT
PGPAGDNPLE LSRIPDENCQ INRYGHFQAT ITIVEGILEV NIIQMTDVLM
PVPWPESSLI DFVVTCQGSI PTEVCTIISD PTCEITQNTV CSPVDVDEMC
LLTVRRTFNG SGTYCVNLTL GDDTSLALTS TLISVPDRDP ASPLRMANSA
LISVGCLAIF VTVISLLVYK KHKEYNPIEN SPGNVVRSKG LSVFLNRAKA
VFFPGNQEKD PLLKNQEFKG VS
UCSC uc003swb.4; human. [Q14956-1];

Category

Human GPNMB ELISA Kit