Human GZMB ELISA Kit

Alternative name
Human C11 ELISA Kit;Human CTLA-1 ELISA Kit;Human cathepsin G-like 1 ELISA Kit;Human CTSGL1 ELISA Kit;Human cytotoxic T-lymphocyte proteinase 2 ELISA Kit;Human Lymphocyte protease ELISA Kit;Human Fragmentin-2 ELISA Kit;Human Granzyme-2 ELISA Kit;Human human lymphocyte protein ELISA Kit;Human HLP ELISA Kit;Human SECT ELISA Kit;Human T-cell serine protease 1-3E ELISA Kit;Human CGL1 ELISA Kit;Human CSPB ELISA Kit;Human CTLA1 ELISA Kit;Human GRB ELISA Kit;Human CCPI ELISA Kit;Human CGL-1 ELISA Kit;Human CSP-B ELISA Kit;Human granzyme B ELISA Kit;Human cytotoxic serine protease B ELISA Kit;Human fragmentin 2 ELISA Kit;Human granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) ELISA Kit;
Catalog
E017436
species
Human
GeneGZMB
SpecificityNatural and recombinant human Granzyme B
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman GZMB ELISA Kit allows for the in vitro quantitative determination of GZMB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human Granzyme B ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Granzyme B has been precoated onto 96-well plates. Standards(NSO, I21-Y247) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Granzyme B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Granzyme B amount of sample captured in plate. Background: Granzyme B is a serine protease that in humans is encoded by the GZMB gene. Granzyme B is expressed by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. CTL and NK cells share the remarkable ability to recognize specific infected target cells. They are thought to protect their host by inducing apoptosis of cells that bear on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.
Human Granzyme B Protein information
Uniprot ID GRAB_HUMAN
Uniprot AC P10144; Q8N1D2; Q9UCC1;
UniGene Hs.1051;
GeneID 3002
KEGG hsa:3002;
Human Granzyme B Protein SEQUENCE
SEQUENCE 247 AA; 27716 MW; C652271918EF24F9 CRC64;
MQPILLLLAF LLLPRADAGE IIGGHEAKPH SRPYMAYLMI WDQKSLKRCG
GFLIRDDFVL TAAHCWGSSI NVTLGAHNIK EQEPTQQFIP VKRPIPHPAY
NPKNFSNDIM LLQLERKAKR TRAVQPLRLP SNKAQVKPGQ TCSVAGWGQT
APLGKHSHTL QEVKMTVQED RKCESDLRHY YDSTIELCVG DPEIKKTSFK
GDSGGPLVCN KVAQGIVSYG RNNGMPPRAC TKVSSFVHWI KKTMKRY
UCSC uc001wps.4; human.;

Category

Human GZMB ELISA Kit