Human DNA damage-inducible transcript 1 protein ELISA Kit;Human DDIT-1 ELISA Kit;Human DDIT1 ELISA Kit;Human GADD45 ELISA Kit;Human growth arrest and DNA damage inducible alpha ELISA Kit;Human growth arrest and DNA damage-inducible protein GADD45 alpha ELISA Kit;Human DNA damage-inducible transcript-1 ELISA Kit;Human growth arrest and DNA-damage-inducible 45 alpha ELISA Kit;
Catalog
E017501
species
Human
GeneGADD45A
SpecificityThis assay has high sensitivity and excellent specificity for detection of human GADD45A. No significant cross-reactivity or interference between human GADD45A and analogues was observed.
SensitivityThe minimum detectable dose of human GADD45A is typically less than 6.25 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m
Intended UseHuman GADD45A ELISA Kit allows for the in vitro quantitative determination of GADD45A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GADD45A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GADD45A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GADD45A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GADD45A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Growth arrest and DNA damage-inducible protein GADD45 alpha Protein information
Uniprot ID
GA45A_HUMAN
Uniprot AC
P24522; Q5TCA7; Q5TCA8;
UniGene
Hs.80409;
GeneID
1647
KEGG
hsa:1647;
Human Growth arrest and DNA damage-inducible protein GADD45 alpha Protein SEQUENCE