Human GDF10 ELISA Kit

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  • Alternative name

    Human GDF-10 ELISA Kit;Human bone morphogenetic protein 3B ELISA Kit;Human BMP-3b ELISA Kit;Human bone-inducing protein ELISA Kit;Human BIP ELISA Kit;Human BMP3B ELISA Kit;Human growth differentiation factor 10 ELISA Kit;Human growth/differentiation factor 10 ELISA Kit;

  • Catalog
    E017521
  • species
    Human
  • GeneGDF10
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human GDF10. No significant cross-reactivity or interference between human GDF10 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human GDF10 is typically less than 5.86 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
  • Intended UseHuman GDF10 ELISA Kit allows for the in vitro quantitative determination of GDF10 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GDF10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GDF10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GDF10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GDF10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Growth/differentiation factor 10 Protein information
  • Uniprot ID GDF10_HUMAN
  • Uniprot AC P55107; Q5VSQ8; Q9UCX6;
  • UniGene Hs.2171;
  • GeneID 2662
  • KEGG hsa:2662;
  • Human Growth/differentiation factor 10 Protein SEQUENCE
  • SEQUENCE 478 AA; 53122 MW; 80AE0FA4C50B23A9 CRC64;

    MAHVPARTSP GPGPQLLLLL LPLFLLLLRD VAGSHRAPAW SALPAAADGL

    QGDRDLQRHP GDAAATLGPS AQDMVAVHMH RLYEKYSRQG ARPGGGNTVR

    SFRARLEVVD QKAVYFFNLT SMQDSEMILT ATFHFYSEPP RWPRALEVLC

    KPRAKNASGR PLPLGPPTRQ HLLFRSLSQN TATQGLLRGA MALAPPPRGL

    WQAKDISPIV KAARRDGELL LSAQLDSEER DPGVPRPSPY APYILVYAND

    LAISEPNSVA VTLQRYDPFP AGDPEPRAAP NNSADPRVRR AAQATGPLQD

    NELPGLDERP PRAHAQHFHK HQLWPSPFRA LKPRPGRKDR RKKGQEVFMA

    ASQVLDFDEK TMQKARRKQW DEPRVCSRRY LKVDFADIGW NEWIISPKSF

    DAYYCAGACE FPMPKIVRPS NHATIQSIVR AVGIIPGIPE PCCVPDKMNS

    LGVLFLDENR NVVLKVYPNM SVDTCACR

  • UCSC uc001jfb.4; human.;



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