Human GDF3 ELISA Kit

Alternative name
Human GDF-3 ELISA Kit;Human KFS3 ELISA Kit;Human MCOP7 ELISA Kit;Human MCOPCB6 ELISA Kit;Human growth differentiation factor 3 ELISA Kit;Human growth/differentiation factor 3 ELISA Kit;
Catalog
E017533
species
Human
GeneGDF3
SpecificityThis assay has high sensitivity and excellent specificity for detection of GDF3. No significant cross-reactivity or interference between GDF3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GDF3 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman GDF3 ELISA Kit allows for the in vitro quantitative determination of GDF3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCancer
Product Description
specificalPrinciple of the assay: GDF3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GDF3. Standards or samples are then added to the microtiter plate wells and GDF3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GDF3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GDF3 are added to each well to "sandwich" the GDF3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GDF3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GDF3 concentration in each sample is interpolated from this standard curve.
Human Growth/differentiation factor 3 Protein information
Uniprot ID GDF3_HUMAN
Uniprot AC Q9NR23; Q8NEJ4;
UniGene Hs.86232;
GeneID 9573
KEGG hsa:9573;
Human Growth/differentiation factor 3 Protein SEQUENCE
SEQUENCE 364 AA; 41387 MW; E0D2EA86E2B73A0B CRC64;
MLRFLPDLAF SFLLILALGQ AVQFQEYVFL QFLGLDKAPS PQKFQPVPYI
LKKIFQDREA AATTGVSRDL CYVKELGVRG NVLRFLPDQG FFLYPKKISQ
ASSCLQKLLY FNLSAIKERE QLTLAQLGLD LGPNSYYNLG PELELALFLV
QEPHVWGQTT PKPGKMFVLR SVPWPQGAVH FNLLDVAKDW NDNPRKNFGL
FLEILVKEDR DSGVNFQPED TCARLRCSLH ASLLVVTLNP DQCHPSRKRR
AAIPVPKLSC KNLCHRHQLF INFRDLGWHK WIIAPKGFMA NYCHGECPFS
LTISLNSSNY AFMQALMHAV DPEIPQAVCI PTKLSPISML YQDNNDNVIL
RHYEDMVVDE CGCG
UCSC uc001qte.4; human.;

Category

Human GDF3 ELISA Kit

Human GDF-3 ELISA Kit;Human KFS3 ELISA Kit;Human MCOP7 ELISA Kit;Human MCOPCB6 ELISA Kit;Human growth differentiation factor 3 ELISA Kit;Human growth/differentiation factor 3 ELISA Kit;