Human GH1 ELISA Kit

Alternative name
Human Growth hormone ELISA Kit;Human GH ELISA Kit;Human GH-N ELISA Kit;Human growth hormone 1 ELISA Kit;Human pituitary growth hormone ELISA Kit;Human GHB5 ELISA Kit;Human GHN ELISA Kit;Human IGHD1B ELISA Kit;Human hGH-N ELISA Kit;Human somatotropin ELISA Kit;Human growth hormone B5 ELISA Kit;
Catalog
E017570
species
Human
GeneGH1
SpecificityNatural and recombinant human GH
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 2pg/ml
Intended UseHuman GH1 ELISA Kit allows for the in vitro quantitative determination of GH1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human GH ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GH has been precoated onto 96-well plates. Standards(E.coli,F27-F217) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GH is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GH amount of sample captured in plate. Background: Growth hormone (GH) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. The human genes for growth hormone (GH), has been located on chromosome 17 in humans. GH replacement of adults with acquired GH deficiency (GHD) results in body composition changes including increases in lean mass and bone mineral density. However, the effects of long-term GH therapy on cognitive function are largely unknown, and there are conflicting data regarding quality of life.
Human Somatotropin Protein information
Uniprot ID SOMA_HUMAN
Uniprot AC P01241; A6NEF6; Q14405; Q16631; Q5EB53; Q9HBZ1; Q9UMJ7; Q9UNL5;
UniGene Hs.655229;
GeneID 2688
KEGG hsa:2688;
Human Somatotropin Protein SEQUENCE
SEQUENCE 217 AA; 24847 MW; 72CC15AF4ED1C51A CRC64;
MATGSRTSLL LAFGLLCLPW LQEGSAFPTI PLSRLFDNAM LRAHRLHQLA
FDTYQEFEEA YIPKEQKYSF LQNPQTSLCF SESIPTPSNR EETQQKSNLE
LLRISLLLIQ SWLEPVQFLR SVFANSLVYG ASDSNVYDLL KDLEEGIQTL
MGRLEDGSPR TGQIFKQTYS KFDTNSHNDD ALLKNYGLLY CFRKDMDKVE
TFLRIVQCRS VEGSCGF
UCSC uc002jdi.4; human. [P01241-1];

Category

Human GH1 ELISA Kit

Human Growth hormone ELISA Kit;Human GH ELISA Kit;Human GH-N ELISA Kit;Human growth hormone 1 ELISA Kit;Human pituitary growth hormone ELISA Kit;Human GHB5 ELISA Kit;Human GHN ELISA Kit;Human IGHD1B ELISA Kit;Human hGH-N ELISA Kit;Human somatotropin ELISA Kit;Human growth hormone B5 ELISA Kit;